Self assembly of acetylcholinesterase on a gold nanoparticles–graphene nanosheet hybrid for organophosphate pesticide detection using polyelectrolyte as a linker
A nanohybrid of gold nanoparticles (Au NPs) and chemically reduced graphene oxide nanosheets (cr-Gs) was synthesized by in situ growth of Au NPs on the surface of graphene nanosheets in the presence of poly(diallyldimethylammonium chloride) (PDDA), which not only improved the dispersion of Au NPs but also stabilized cholinesterase with high activity and loading efficiency. The obtained nanohybrid was characterized by TEM, XRD, XPS, and electrochemistry. Then an enzyme nanoassembly (AChE/Au NPs/cr-Gs) was prepared by self-assembling acetylcholinesterase (AChE) on Au NP/cr-Gs nanohybrid. An electrochemical sensor based on AChE/Au NPs/cr-Gs was further developed for ultrasensitive detection of organophosphate pesticide. The results demonstrate that the developed approach provides a promising strategy to improve the sensitivity and enzyme activity of electrochemical biosensors.
- Research Organization:
- Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC05-76RL01830
- OSTI ID:
- 1012291
- Report Number(s):
- PNNL-SA-78018; 39985
- Journal Information:
- Journal of Materials Chemistry, 21(14):5319-5325, Journal Name: Journal of Materials Chemistry, 21(14):5319-5325 Journal Issue: 14 Vol. 21; ISSN JMACEP; ISSN 0959-9428
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
60 APPLIED LIFE SCIENCES
CHOLINESTERASE
DETECTION
EFFICIENCY
ELECTROCHEMISTRY
ENZYME ACTIVITY
ENZYMES
Environmental Molecular Sciences Laboratory
GOLD
OXIDES
PESTICIDES
SENSITIVITY
SENSORS
X-RAY DIFFRACTION
X-RAY PHOTOELECTRON SPECTROSCOPY
biosensor
enzyme
gold nanoparticles
graphene