Biological sample preparation and {sup 41}Ca AMS measurement at LLNL
- Lawrence Livermore National Lab., CA (United States). Center for Accelerator Mass Spectrometry
- Iowa State Univ., Ames, IA (United States). Dept. of Food Science and Human Nutrition
- Univ. of California, Berkeley, CA (United States). Dept. of Nutritional Sciences
Calcium metabolism in biology may be better understood by the use of {sup 41}Ca labels, although detection by accelerator mass spectrometry (AMS) is required. Methodologies for preparation of urine samples and subsequent AMS measurement were investigated. Novel attempts at preparing CaH{sub 2} were unsuccessful, but CaF{sub 2} of sufficient purity could be produced by precipitation of calcium from urine as oxalate, followed by separation of calcium by cation exchange chromatography and washing the CaF{sub 2} precipitate. The presence of some remaining impurities could be compensated for by selecting the appropriate accelerated ion charge state for AMS. The use of projectile x rays for isobar discrimination was explored as an alternative to the conventional dE/dx device.
- Research Organization:
- Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
- Sponsoring Organization:
- USDOE, Washington, DC (United States)
- DOE Contract Number:
- W-7405-ENG-48
- OSTI ID:
- 10118276
- Report Number(s):
- UCRL-JC-116670; CONF-941129-14; ON: DE95006439; TRN: AHC29508%%122
- Resource Relation:
- Conference: 13. international conference on the application of accelerators in research and industry,Denton, TX (United States),7-10 Nov 1994; Other Information: PBD: 10 Oct 1994
- Country of Publication:
- United States
- Language:
- English
Similar Records
Ionic conductivity and the formation of cubic CaH{sub 2} in the LiBH{sub 4}–Ca(BH{sub 4}){sub 2} composite
Isolating /sup 241/Am from waste solutions containing Al, Ca, Fe, and Cr