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Phase-sensitive flow cytometry: New technology for analyzing biochemical, functional, and structural features in fluorochrome- labeled cells/particles

Conference ·
OSTI ID:10107449
Flow cytometry (FCM) instruments rapidly measure biochemical, functional, and cytological properties of individual cells and macromolecular components, e.g., chromosomes, for clinical diagnostic medicine and biomedical and envirorunental research applications. These measurements are based on labeling cells with multiple fluorochromes for correlated analysis of macromolecules, such as DNA RNA, protein, and cell-surface receptors. This report describes the development of a phase-sensitive flow cytometer that provides unique capabilities for making laser-excited, phase-resolved measurements on fluorochrome-labelled cells and particles.
Research Organization:
Los Alamos National Lab., NM (United States)
Sponsoring Organization:
USDOE, Washington, DC (United States); Department of Health and Human Services, Washington, DC (United States)
DOE Contract Number:
W-7405-ENG-36
OSTI ID:
10107449
Report Number(s):
LA-UR--93-3960; CONF-940341--2; ON: DE94003960; CNN: Grant P41-RR01315; R01-RR07855
Country of Publication:
United States
Language:
English

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