Genetics of thermophilic bacteria. Final progress report, May 1, 1984--April 30, 1991

Technical Report · Tue Dec 31 04:00:00 EST 1991

DOI:https://doi.org/10.2172/10107076· OSTI ID:10107076

Welker, N E

Organisms adapted to high temperature have evolved a variety of unique solutions to the biochemical problems imposed by this environment. Adaptation is commonly used to describe the biochemical properties of organisms which have become adapted to their environment (genetic adaptation). It can also mean the direct response-at the cellular level-of an organism to changes in temperature (physiological adaptation). Thermophilic bacilli (strains of Bacillus stearothermophilus) can exhibit a variety of biochemical adaptations in response to changes in temperature. These include changes in the composition and stability of the membrane, metabolic potential, the transport of amino acids, regulatory mechanisms, ribose methylation of tRNA, protein thermostability, and nutritional requirements. The objectives of the research were to develop efficient and reliable genetic systems to analyze and manipulate B. Stearothermophilus, and to use these systems initiate a biochemical, molecular, and genetic investigations of genes that are required for growth at high temperature.

• Research Organization: Northwestern Univ., Evanston, IL (United States). Dept. of Biochemistry, Molecular Biology and Cell Biology
• Sponsoring Organization: USDOE, Washington, DC (United States)
• DOE Contract Number: FG02-84ER13204
• OSTI ID: 10107076
• Report Number(s): DOE/ER/13204--6; ON: DE92004033
• Country of Publication: United States
• Language: English

Related Subjects

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59 BASIC BIOLOGICAL SCIENCES
BACILLUS
BIOCHEMISTRY
BIOLOGICAL ADAPTATION
DNA SEQUENCING
DNA-CLONING
GENE OPERONS
GENE REGULATION
GENETIC MAPPING
GENETICS
MICROBIOLOGY
MOLECULAR BIOLOGY
MUTANTS
PLASMIDS
PROGRESS REPORT
TEMPERATURE DEPENDENCE
THERMAL STRESSES
TRANSPOSONS