Suppression of ICE and Apoptosis in Mammary Epithelial Cells by Extracellular Matrix

Journal Article · Thu Dec 01 00:00:00 EST 1994 · Science

OSTI ID:1009838

Boudreau, Nancy; Sympson, C J; Werb, Zena; Bissell, Mina J

Apoptosis (programmed cell death) plays a major role in development and tissue regeneration. Basement membrane extracellular matrix (ECM), but not fibronectin or collagen, was shown to suppress apoptosis of mammary epithelial cells in tissue culture and in vivo. Apoptosis was induced by antibodies to beta 1 integrins or by overexpression of stromelysin-1, which degrades ECM. Expression of interleukin-1 beta converting enzyme (ICE) correlated with the loss of ECM, and inhibitors of ICE activity prevented apoptosis. These results suggest that ECM regulates apoptosis in mammary epithelial cells through an integrin-dependent negative regulation of ICE expression.

• Research Organization: Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
• Sponsoring Organization: Life Sciences Division
• DOE Contract Number: DE-AC02-05CH11231; CA 57621, DE-AC-03-76-SF00098, DE-AC-03-76-SF10102
• OSTI ID: 1009838
• Report Number(s): LBNL-4333E; TRN: US201106%%1028
• Journal Information: Science, Vol. 267, Issue 5199
• Country of Publication: United States
• Language: English

Related Subjects

59
ANTIBODIES
APOPTOSIS
BASEMENTS
COLLAGEN
DEATH
ENZYMES
IN VIVO
MEMBRANES
REGENERATION
REGULATIONS
TISSUE CULTURES