Structural Basis for Imipenim Inhibition of Class C [beta]-Lactamases
Journal Article
·
· Antimicrob. Agents Ch.
OSTI ID:1008815
- NWU
To determine how imipenem inhibits the class C {beta}-lactamase AmpC, the X-ray crystal structure of the acyl-enzyme complex was determined to a resolution of 1.80 {angstrom}. In the complex, the lactam carbonyl oxygen of imipenem has flipped by approximately 180{sup o} compared to its expected position; the electrophilic acyl center is thus displaced from the point of hydrolytic attack. This conformation resembles that of imipenem bound to the class A enzyme TEM-1 but is different from that of moxalactam bound to AmpC.
- Research Organization:
- Advanced Photon Source (APS), Argonne National Laboratory (ANL), Argonne, IL (US)
- Sponsoring Organization:
- USDOE
- OSTI ID:
- 1008815
- Journal Information:
- Antimicrob. Agents Ch., Journal Name: Antimicrob. Agents Ch. Journal Issue: (12) ; 12, 2002 Vol. 46; ISSN 0066-4804; ISSN AMACCQ
- Country of Publication:
- United States
- Language:
- ENGLISH
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