Structural Insight into the Dioxygenation of Nitroarene Compounds: the Crystal Structure of Nitrobenzene Dioxygenase
- Uppsala
Nitroaromatic compounds are used extensively in many industrial processes and have been released into the environment where they are considered environmental pollutants. Nitroaromatic compounds, in general, are resistant to oxidative attack due to the electron-withdrawing nature of the nitro groups and the stability of the benzene ring. However, the bacterium Comamonas sp. strain JS765 can grow with nitrobenzene as a sole source of carbon, nitrogen and energy. Biodegradation is initiated by the nitrobenzene dioxygenase (NBDO) system. We have determined the structure of NBDO, which has a hetero-hexameric structure similar to that of several other Rieske non-heme iron dioxygenases. The catalytic subunit contains a Rieske iron-sulfur center and an active-site mononuclear iron atom. The structures of complexes with substrates nitrobenzene and 3-nitrotoluene reveal the structural basis for its activity with nitroarenes. The substrate pocket contains an asparagine residue that forms a hydrogen bond to the nitro-group of the substrate, and orients the substrate in relation to the active-site mononuclear iron atom, positioning the molecule for oxidation at the nitro-substituted carbon.
- Research Organization:
- Advanced Photon Source (APS), Argonne National Laboratory (ANL), Argonne, IL (US)
- Sponsoring Organization:
- USDOE
- OSTI ID:
- 1008659
- Journal Information:
- J. Mol. Biol., Journal Name: J. Mol. Biol. Journal Issue: (5) ; 2005 Vol. 348; ISSN JMOBAK; ISSN 0022-2836
- Country of Publication:
- United States
- Language:
- ENGLISH
Similar Records
Structures of the multicomponent Rieske non-heme iron toluene 2, 3-dioxygenase enzyme system
Structures of the Multicomponent Rieske Non-Heme Iron Toluene 2,3-Dioxygenase Enzyme System
Structural Investigations of the Ferredoxin and Terminal Oxygenase Components of the biphenyl 2,3-dioxygenase from Sphingobium yanoikuyae B1
Journal Article
·
Wed Dec 31 23:00:00 EST 2008
· Acta Crystallographica. Section D: Biological Crystallography
·
OSTI ID:22347992
Structures of the Multicomponent Rieske Non-Heme Iron Toluene 2,3-Dioxygenase Enzyme System
Journal Article
·
Wed Dec 31 23:00:00 EST 2008
· Acta Crystallographica Section D: Biological Crystallography
·
OSTI ID:980573
Structural Investigations of the Ferredoxin and Terminal Oxygenase Components of the biphenyl 2,3-dioxygenase from Sphingobium yanoikuyae B1
Journal Article
·
Sun Dec 31 23:00:00 EST 2006
· BMC Structural Biology
·
OSTI ID:930655