Structure of Escherichia coli ribonucleotide reductase R2 in space group P6[subscript 1]22
Journal Article
·
· Acta Crystallogr. D
OSTI ID:1008633
- NWU
A new crystal form of wild-type ribonucleotide reductase R2 from Escherichia coli was obtained. Crystals grow in space group P6{sub 1}22 with one R2 monomer in the asymmetric unit. A twofold crystallographic symmetry axis generates the physiological dimeric form of R2. Co-crystallization with CoCl{sub 2} or MnCl{sub 2} results in full occupancy of the dinuclear metal site. The structure of the Mn{sup II}-loaded form was determined to 2.6 {angstrom} resolution by molecular replacement. The crystallization conditions, backbone conformation, crystal-packing interactions and metal centers are compared with those of previously determined crystal forms.
- Research Organization:
- Advanced Photon Source (APS), Argonne National Laboratory (ANL), Argonne, IL (US)
- Sponsoring Organization:
- USDOE
- OSTI ID:
- 1008633
- Journal Information:
- Acta Crystallogr. D, Journal Name: Acta Crystallogr. D Journal Issue: (12) ; 2005 Vol. 61; ISSN 0907-4449
- Country of Publication:
- United States
- Language:
- ENGLISH
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