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Thermal tolerant mannanase from acidothermus cellulolyticus

Patent ·
OSTI ID:1007922

The invention provides a thermal tolerant mannanase that is a member of the glycoside hydrolase family. The invention further discloses this mannanase as ManA. ManA has been isolated and characterized from Acidothermus cellulolyticus. The invention further provides recombinant forms of the identified ManA. Methods of making ManA polypeptides, including fusions, variants, and derivatives, are also disclosed. Methods of using mannanase A, including for the processing of food and for use in food stuffs as bulking agents and the like, are also disclosed.

Research Organization:
National Renewable Energy Laboratory (NREL), Golden, CO (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC36-99GO10337
Assignee:
Midwest Research Institute (Kansas City, MO)
Patent Number(s):
7,112,429
Application Number:
09/917,378
OSTI ID:
1007922
Country of Publication:
United States
Language:
English

References (5)

Ultra-Thermostable Cellulases From Acidothermus cellulolyticus: Comparison of Temperature Optima with Previously Reported Cellulases journal August 1989
The beta-mannanase from "Caldocellum saccharolyticum" is part of a multidomain enzyme. journal January 1992
Synergism Between Purified Bacterial and Fungal Cellulases book May 1996
Purification and some properties of ?-mannanase fromPolyporus versicolor journal September 1990
A Gene Encoding a Novel Multidomain β-1,4-Mannanase from Caldibacillus cellulovorans and Action of the Recombinant Enzyme on Kraft Pulp journal February 2000

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