Structure of G[alpha subscript q]-p63RhoGEF-RhoA Complex Reveals a Pathway for the Activation of RhoA by GPCRs
- HeidelbergU
The guanine nucleotide exchange factor p63RhoGEF is an effector of the heterotrimeric guanine nucleotide-binding protein (G protein) G{alpha}{sub q} and thereby links G{alpha}{sub q}-coupled receptors (GPCRs) to the activation of the small-molecular-weight G protein RhoA. We determined the crystal structure of the G{alpha}{sub q}-p63RhoGEF-RhoA complex, detailing the interactions of G{alpha}{sub q} with the Dbl and pleckstrin homology (DH and PH) domains of p63RhoGEF. These interactions involve the effector-binding site and the C-terminal region of G{alpha}{sub q} and appear to relieve autoinhibition of the catalytic DH domain by the PH domain. Trio, Duet, and p63RhoGEF are shown to constitute a family of G{alpha}{sub q} effectors that appear to activate RhoA both in vitro and in intact cells. We propose that this structure represents the crux of an ancient signal transduction pathway that is expected to be important in an array of physiological processes.
- Research Organization:
- Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
- Sponsoring Organization:
- USDOE
- OSTI ID:
- 1007639
- Journal Information:
- Science, Vol. 318, Issue 12, 2007; ISSN 0193-4511
- Country of Publication:
- United States
- Language:
- ENGLISH
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