Controlled Loading of Building Blocks into Temporary Self-Assembled Scaffolds for Directed Assembly of Organic Nanostructures

Journal Article · Mon Dec 08 04:00:00 EST 2008 · Langmuir

DOI:https://doi.org/10.1021/la801755b· OSTI ID:1006990

Banner, L Todd; Danila, Delia C; Sharpe, Katie; Durkin, Melissa; Clayton, Benjamin; Anderson, Ben; Richter, Andrew; Pinkhassik, Eugene ^[1]

---

1. Valparaiso

Using temporary self-assembled scaffolds to preorganize building blocks is a potentially powerful method for the synthesis of organic nanostructures with programmed shapes. We examined the underlying phenomena governing the loading of hydrophobic monomers into lipid bilayer interior and demonstrated successful control of the amount and ratio of loaded monomers. When excess styrene derivatives or acrylates were added to the aqueous solution of unilamellar liposomes made from saturated phospholipids, most loading occurs within the first few hours. Dynamic light scattering and transmission electron microscopy revealed no evidence of aggregation caused by monomers. Bilayers appeared to have a certain capacity for accommodating monomers. The total volume of loaded monomers is independent of monomer structure. X-ray scattering showed the increase in bilayer thickness consistent with loading monomers into bilayer interior. Loading kinetics is inversely proportional to the hydrophobicity and size of monomers. Loading and extraction kinetic data suggest that crossing the polar heads region is the rate limiting step. Consideration of loading kinetics and multiple equilibria are important for achieving reproducible monomer loading. The total amount of monomers loaded into the bilayer can be controlled by the loading time or length of hydrophobic lipid tails. The ratio of loaded monomers can be varied by changing the ratio of monomers used for loading or by the time-controlled replacement of a preloaded monomer. Understanding and controlling the loading of monomers into bilayers contributes to the directed assembly of organic nanostructures.

• Research Organization: Advanced Photon Source (APS), Argonne National Laboratory (ANL), Argonne, IL (US)
• Sponsoring Organization: USDOE
• OSTI ID: 1006990
• Journal Information: Langmuir, Journal Name: Langmuir Journal Issue: (20) ; 2008 Vol. 24; ISSN LANGD5; ISSN 0743-7463
• Country of Publication: United States
• Language: ENGLISH

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
77 NANOSCIENCE AND NANOTECHNOLOGY
ACRYLATES
AGGLOMERATION
AQUEOUS SOLUTIONS
BUILDINGS
CAPACITY
CONTROL
DATA
DYNAMICS
EXTRACTION
KINETICS
LENGTH
LIGHT SCATTERING
LIPIDS
LIPOSOMES
LOADING
MONOMERS
NANOSTRUCTURES
PHOSPHOLIPIDS
SCATTERING
SIZE
STYRENE
SYNTHESIS
THICKNESS
TRANSMISSION ELECTRON MICROSCOPY