Structures of the G85R Variant of SOD1 in Familial Amyotrophic Lateral Sclerosis

Journal Article · Mon Jul 21 04:00:00 EDT 2008 · J. Biol. Chem.

DOI:https://doi.org/10.1074/jbc.M801522200· OSTI ID:1006667

Cao, Xiaohang; Antonyuk, Svetlana V; Seetharaman, Sai V; Whitson, Lisa J; Taylor, Alexander B; Holloway, Stephen P; Strange, Richard W; Doucette, Peter A; Valentine, Joan Selverstone; Tiwari, Ashutosh; Hayward, Lawrence J; Padua, Shelby; Cohlberg, Jeffrey A; Hasnain, S Samar; Hart, P John ^[1]

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1. Texas-HSC

Mutations in the gene encoding human copper-zinc superoxide dismutase (SOD1) cause a dominant form of the progressive neurodegenerative disease amyotrophic lateral sclerosis. Transgenic mice expressing the human G85R SOD1 variant develop paralytic symptoms concomitant with the appearance of SOD1-enriched proteinaceous inclusions in their neural tissues. The process(es) through which misfolding or aggregation of G85R SOD1 induces motor neuron toxicity is not understood. Here we present structures of the human G85R SOD1 variant determined by single crystal x-ray diffraction. Alterations in structure of the metal-binding loop elements relative to the wild type enzyme suggest a molecular basis for the metal ion deficiency of the G85R SOD1 protein observed in the central nervous system of transgenic mice and in purified recombinant G85R SOD1. These findings support the notion that metal-deficient and/or disulfide-reduced mutant SOD1 species contribute to toxicity in SOD1-linked amyotrophic lateral sclerosis.

• Research Organization: Advanced Photon Source (APS), Argonne National Laboratory (ANL), Argonne, IL (US)
• Sponsoring Organization: USDOE
• OSTI ID: 1006667
• Journal Information: J. Biol. Chem., Journal Name: J. Biol. Chem. Journal Issue: (23) ; 06, 2008 Vol. 283; ISSN JBCHA3; ISSN 0021-9258
• Country of Publication: United States
• Language: ENGLISH

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
AGGLOMERATION
CENTRAL NERVOUS SYSTEM
DISEASES
ELEMENTS
ENZYMES
GENES
INCLUSIONS
IONS
METALS
MONOCRYSTALS
MOTORS
MUTANTS
MUTATIONS
NERVE CELLS
PROTEINS
SUPEROXIDE DISMUTASE
SYMPTOMS
TOXICITY
TRANSGENIC MICE
X-RAY DIFFRACTION