Inhibitor-complexed Structures of the Cytochrome bc[subscript 1] from the Photosynthetic Bacterium Rhodobacter sphaeroides
- NCI
The cytochrome bc{sub 1} complex (bc{sub 1}) is a major contributor to the proton motive force across the membrane by coupling electron transfer to proton translocation. The crystal structures of wild type and mutant bc{sub 1} complexes from the photosynthetic purple bacterium Rhodobacter sphaeroides (Rsbc{sub 1}), stabilized with the quinol oxidation (Q{sub P}) site inhibitor stigmatellin alone or in combination with the quinone reduction (Q{sub N}) site inhibitor antimycin, were determined. The high quality electron density permitted assignments of a new metal-binding site to the cytochrome c1 subunit and a number of lipid and detergent molecules. Structural differences between Rsbc{sub 1} and its mitochondrial counterparts are mostly extra membranous and provide a basis for understanding the function of the predominantly longer sequences in the bacterial subunits. Functional implications for the bc{sub 1} complex are derived from analyses of 10 independent molecules in various crystal forms and from comparisons with mitochondrial complexes.
- Research Organization:
- Advanced Photon Source (APS), Argonne National Laboratory (ANL), Argonne, IL (US)
- Sponsoring Organization:
- USDOE
- OSTI ID:
- 1006507
- Journal Information:
- J. Biol. Chem., Journal Name: J. Biol. Chem. Journal Issue: (5) ; 02, 2008 Vol. 283; ISSN JBCHA3; ISSN 0021-9258
- Country of Publication:
- United States
- Language:
- ENGLISH
Similar Records
Three-Dimensional Structure of the Rhodobacter sphaeroides RC-LH1-PufX Complex: Dimerization and Quinone Channels Promoted by PufX
Crystal structure of bacterial cytochrome bc1 in complex with azoxystrobin reveals a conformational switch of the Rieske iron–sulfur protein subunit
The Interaction of the Rieske Iron-Sulfur Protein with Occupants of the Q(o)-site of the bc(1) Complex, Probed by Electron Spin Echo Envelope Modulation
Journal Article
·
Wed Oct 16 00:00:00 EDT 2013
· Biochemistry
·
OSTI ID:1097031
Crystal structure of bacterial cytochrome bc1 in complex with azoxystrobin reveals a conformational switch of the Rieske iron–sulfur protein subunit
Journal Article
·
Sun Jun 09 20:00:00 EDT 2019
· Journal of Biological Chemistry
·
OSTI ID:1562697
The Interaction of the Rieske Iron-Sulfur Protein with Occupants of the Q(o)-site of the bc(1) Complex, Probed by Electron Spin Echo Envelope Modulation
Journal Article
·
Wed Feb 14 23:00:00 EST 2001
· Journal of Biological Chemistry
·
OSTI ID:15005606