Expression, purification, crystallization and preliminary X-ray studies of Vibrio cholerae pseudopilin EpsH
Journal Article
·
· Acta Crystallogr. F
EpsH is a minor pseudopilin protein of the Vibrio cholerae type II secretion system. A truncated form of EpsH with a C-terminal noncleavable His tag was constructed and expressed in Escherichia coli, purified and crystallized by sitting-drop vapor diffusion. A complete data set was collected to 1.71 {angstrom} resolution. The crystals belonged to space group P2{sub 1}2{sub 1}2{sub 1}, with unit-cell parameters a = 53.39, b = 71.11, c = 84.64 {angstrom}. There were two protein molecules in the asymmetric unit, which gave a Matthews coefficient V{sub M} of 2.1 {angstrom}{sup 3} Da{sup -1}, corresponding to 41.5% solvent content.
- Research Organization:
- Argonne National Lab. (ANL), Argonne, IL (United States)
- Sponsoring Organization:
- USDOE
- OSTI ID:
- 1006065
- Journal Information:
- Acta Crystallogr. F, Vol. 65, Issue (7) ; 07, 2009; ISSN 1744-3091
- Country of Publication:
- United States
- Language:
- ENGLISH
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