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Electron Density Guided Fragment-Based Lead Discovery of Ketohexokinase Inhibitors

Journal Article · · J. Med. Chem.
DOI:https://doi.org/10.1021/jm100677s· OSTI ID:1002926

A fragment-based drug design paradigm has been successfully applied in the discovery of lead series of ketohexokinase inhibitors. The paradigm consists of three iterations of design, synthesis, and X-ray crystallographic screening to progress low molecular weight fragments to leadlike compounds. Applying electron density of fragments within the protein binding site as defined by X-ray crystallography, one can generate target specific leads without the use of affinity data. Our approach contrasts with most fragment-based drug design methodology where solution activity is a main design guide. Herein we describe the discovery of submicromolar ketohexokinase inhibitors with promising druglike properties.

Research Organization:
Advanced Photon Source (APS), Argonne National Laboratory (ANL), Argonne, IL (US)
Sponsoring Organization:
USDOE
OSTI ID:
1002926
Journal Information:
J. Med. Chem., Journal Name: J. Med. Chem. Journal Issue: (22) ; 11, 2010 Vol. 53; ISSN JMCMAR; ISSN 0022-2623
Country of Publication:
United States
Language:
ENGLISH

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