Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of macrophage growth locus A (MglA) protein from Francisella tularensis

Journal Article · Wed Nov 03 00:00:00 EDT 2010 · Acta Crystallogr. F

DOI:https://doi.org/10.1107/S1744309110009711· OSTI ID:1002378

Subburaman, P; Austin, B P; Shaw, G X; Waugh, D S; Ji, X ^[1]

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1. NCI

Francisella tularensis, a potential bioweapon, causes a rare infectious disease called tularemia in humans and animals. The macrophage growth locus A (MglA) protein from F. tularensis associates with RNA polymerase to positively regulate the expression of multiple virulence factors that are required for its survival and replication within macrophages. The MglA protein was overproduced in Escherichia coli, purified and crystallized. The crystals diffracted to 7.5 {angstrom} resolution at the Advanced Photon Source, Argonne National Laboratory and belonged to the hexagonal space group P6{sub 1} or P6{sub 5}, with unit-cell parameters a = b = 125, c = 54 {angstrom}.

• Research Organization: Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
• Sponsoring Organization: USDOE
• OSTI ID: 1002378
• Journal Information: Acta Crystallogr. F, Vol. 66, Issue (5) ; 05, 2010
• Country of Publication: United States
• Language: ENGLISH

Related Subjects

45 MILITARY TECHNOLOGY, WEAPONRY, AND NATIONAL DEFENSE
99 GENERAL AND MISCELLANEOUS//MATHEMATICS, COMPUTING, AND INFORMATION SCIENCE
BIOLOGICAL WARFARE AGENTS
CLONING
CRYSTALLIZATION
ESCHERICHIA COLI
INFECTIOUS DISEASES
MACROPHAGES
PROTEINS
PURIFICATION
RESOLUTION
RNA POLYMERASES
VIRULENCE
X-RAY DIFFRACTION