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Title: Monitoring molecular interactions using photon arrival-time interval distribution analysis

Abstract

A method for analyzing/monitoring the properties of species that are labeled with fluorophores. A detector is used to detect photons emitted from species that are labeled with one or more fluorophores and located in a confocal detection volume. The arrival time of each of the photons is determined. The interval of time between various photon pairs is then determined to provide photon pair intervals. The number of photons that have arrival times within the photon pair intervals is also determined. The photon pair intervals are then used in combination with the corresponding counts of intervening photons to analyze properties and interactions of the molecules including brightness, concentration, coincidence and transit time. The method can be used for analyzing single photon streams and multiple photon streams.

Inventors:
 [1];  [2]
  1. Livermore, CA
  2. Los Angels, CA
Publication Date:
Research Org.:
The Regents of the University of California (Oakland, CA)
Sponsoring Org.:
USDOE
OSTI Identifier:
1001991
Patent Number(s):
7,599,059
Application Number:
10/521,632
Assignee:
The Regents of the University of California (Oakland, CA) OAK
DOE Contract Number:  
AC03-76SF00098
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS

Citation Formats

Laurence, Ted A, and Weiss, Shimon. Monitoring molecular interactions using photon arrival-time interval distribution analysis. United States: N. p., 2009. Web.
Laurence, Ted A, & Weiss, Shimon. Monitoring molecular interactions using photon arrival-time interval distribution analysis. United States.
Laurence, Ted A, and Weiss, Shimon. Tue . "Monitoring molecular interactions using photon arrival-time interval distribution analysis". United States. https://www.osti.gov/servlets/purl/1001991.
@article{osti_1001991,
title = {Monitoring molecular interactions using photon arrival-time interval distribution analysis},
author = {Laurence, Ted A and Weiss, Shimon},
abstractNote = {A method for analyzing/monitoring the properties of species that are labeled with fluorophores. A detector is used to detect photons emitted from species that are labeled with one or more fluorophores and located in a confocal detection volume. The arrival time of each of the photons is determined. The interval of time between various photon pairs is then determined to provide photon pair intervals. The number of photons that have arrival times within the photon pair intervals is also determined. The photon pair intervals are then used in combination with the corresponding counts of intervening photons to analyze properties and interactions of the molecules including brightness, concentration, coincidence and transit time. The method can be used for analyzing single photon streams and multiple photon streams.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2009},
month = {10}
}

Patent:

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