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Title: Involvement of Shewanella oneidensis MR-1 LuxS in Biofilm Development and Sulfur Metabolism

Abstract

The role of LuxS in Shewanella oneidensis MR-1 has been examined by transcriptomic profiling, biochemical, and physiological experiments. The results indicate that a mutation in luxS alters biofilm development, not by altering quorum-sensing abilities but by disrupting the activated methyl cycle (AMC). The S. oneidensis wild type can produce a luminescence response in the AI-2 reporter strain Vibrio harveyi MM32. This luminescence response is abolished upon the deletion of luxS. The deletion of luxS also alters biofilm formations in static and flowthrough conditions. Genetic complementation restores the mutant biofilm defect, but the addition of synthetic AI-2 has no effect. These results suggest that AI-2 is not used as a quorum-sensing signal to regulate biofilm development in S. oneidensis. Growth on various sulfur sources was examined because of the involvement of LuxS in the AMC. A mutation in luxS produced a reduced ability to grow with methionine as the sole sulfur source. Methionine is a key metabolite used in the AMC to produce a methyl source in the cell and to recycle homocysteine. These data suggest that LuxS is important to metabolizing methionine and the AMC in S. oneidensis.

Authors:
; ; ; ; ; ;
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
Sponsoring Org.:
USDOE
OSTI Identifier:
1001529
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Journal Name:
Applied and Environmental Microbiology, 75(5):1301-1307
Additional Journal Information:
Journal Volume: 75; Journal Issue: 5; Journal ID: ISSN 0099-2240
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 99 GENERAL AND MISCELLANEOUS//MATHEMATICS, COMPUTING, AND INFORMATION SCIENCE; GENETICS; HOMOCYSTEINE; LUMINESCENCE; METABOLISM; METABOLITES; METHIONINE; MUTANTS; MUTATIONS; STRAINS; SULFUR; Environmental Molecular Sciences Laboratory

Citation Formats

Learman, Deric R, Yi, Haakrho, Brown, Steven D, Martin, Stanton L, Geesey, Gill G, Stevens, Ann M, and Hochella, Michael F. Involvement of Shewanella oneidensis MR-1 LuxS in Biofilm Development and Sulfur Metabolism. United States: N. p., 2009. Web. doi:10.1128/AEM.01393-08.
Learman, Deric R, Yi, Haakrho, Brown, Steven D, Martin, Stanton L, Geesey, Gill G, Stevens, Ann M, & Hochella, Michael F. Involvement of Shewanella oneidensis MR-1 LuxS in Biofilm Development and Sulfur Metabolism. United States. https://doi.org/10.1128/AEM.01393-08
Learman, Deric R, Yi, Haakrho, Brown, Steven D, Martin, Stanton L, Geesey, Gill G, Stevens, Ann M, and Hochella, Michael F. 2009. "Involvement of Shewanella oneidensis MR-1 LuxS in Biofilm Development and Sulfur Metabolism". United States. https://doi.org/10.1128/AEM.01393-08.
@article{osti_1001529,
title = {Involvement of Shewanella oneidensis MR-1 LuxS in Biofilm Development and Sulfur Metabolism},
author = {Learman, Deric R and Yi, Haakrho and Brown, Steven D and Martin, Stanton L and Geesey, Gill G and Stevens, Ann M and Hochella, Michael F},
abstractNote = {The role of LuxS in Shewanella oneidensis MR-1 has been examined by transcriptomic profiling, biochemical, and physiological experiments. The results indicate that a mutation in luxS alters biofilm development, not by altering quorum-sensing abilities but by disrupting the activated methyl cycle (AMC). The S. oneidensis wild type can produce a luminescence response in the AI-2 reporter strain Vibrio harveyi MM32. This luminescence response is abolished upon the deletion of luxS. The deletion of luxS also alters biofilm formations in static and flowthrough conditions. Genetic complementation restores the mutant biofilm defect, but the addition of synthetic AI-2 has no effect. These results suggest that AI-2 is not used as a quorum-sensing signal to regulate biofilm development in S. oneidensis. Growth on various sulfur sources was examined because of the involvement of LuxS in the AMC. A mutation in luxS produced a reduced ability to grow with methionine as the sole sulfur source. Methionine is a key metabolite used in the AMC to produce a methyl source in the cell and to recycle homocysteine. These data suggest that LuxS is important to metabolizing methionine and the AMC in S. oneidensis.},
doi = {10.1128/AEM.01393-08},
url = {https://www.osti.gov/biblio/1001529}, journal = {Applied and Environmental Microbiology, 75(5):1301-1307},
issn = {0099-2240},
number = 5,
volume = 75,
place = {United States},
year = {Mon Jan 05 00:00:00 EST 2009},
month = {Mon Jan 05 00:00:00 EST 2009}
}