Identification of Molecular and Cellular Responses of Desulfovibrio vulgaris Biofilms under Culture Conditions Relevant to Field Conditions for Bioreduction of

Technical Report · Thu Jun 01 00:00:00 EDT 2006

DOI:https://doi.org/10.2172/896801· OSTI ID:896801

Wall, Judy D

Desulfovibrio vulgaris Hildenborough forms biofilms under stress and nutrient limitation conditions. Analysis of the transcriptional responses to acid exposure suggested that genes encoding arginine and polyamine biosynthesis were increased in expression. A literature search showed that polyamines had been suggested to stimulate biofilm formation in some bacteria. Therefore, biofilm formation by D. vulgaris was then examined. Two different assay methods were used to estimate the biofilm formation. First, the classical crystal violet stainable material attached to glass test tubes was measured. Second protein attached to the test tube sides and especially precipitated at the bottom was measured.

• Research Organization: University of Missouri, Columbia, MO
• Sponsoring Organization: USDOE Office of Science (SC)
• OSTI ID: 896801
• Report Number(s): ERSD-1026866b-2006; R&D Project: ERSD 1026866; TRN: US200704%%107
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
54 ENVIRONMENTAL SCIENCES
ARGININE
BACTERIA
BIOSYNTHESIS
DESULFOVIBRIO
GENES
GLASS
METHYL VIOLET
NUTRIENTS
PROTEINS