Cytoplasmic bacteriophage display system
- Stony Brook, NY
- Setauket, NY
Disclosed are display vectors comprising DNA encoding a portion of a structural protein from a cytoplasmic bacteriophage, joined covalently to a protein or peptide of interest. Exemplified are display vectors wherein the structural protein is the T7 bacteriophage capsid protein. More specifically, in the exemplified display vectors the C-terminal amino acid residue of the portion of the capsid protein is joined to the N-terminal residue of the protein or peptide of interest. The portion of the T7 capsid protein exemplified comprises an N-terminal portion corresponding to form 10B of the T7 capsid protein. The display vectors are useful for high copy number display or lower copy number display (with larger fusion). Compositions of the type described herein are useful in connection with methods for producing a virus displaying a protein or peptide of interest.
- Research Organization:
- Associated Universities, Inc., Upton, NY (United States)
- DOE Contract Number:
- AC02-76CH00016
- Assignee:
- Associated Universities Inc. (Washington, DC)
- Patent Number(s):
- US 5766905
- Application Number:
- 08/664,449
- OSTI ID:
- 871626
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
bacteriophage
display
disclosed
vectors
comprising
dna
encoding
portion
structural
protein
joined
covalently
peptide
exemplified
t7
capsid
specifically
c-terminal
amino
acid
residue
n-terminal
comprises
corresponding
form
10b
useful
copy
larger
fusion
compositions
type
described
connection
methods
producing
virus
displaying
amino acid
dna encoding
type described
display vectors
acid residue
structural protein
cytoplasmic bacteriophage
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