Development and function of membrane systems in plant tissue. Annual technical progress report, 15 September 1981-15 August 1982
Over the past 11 months we have continued investigation of ion transport mechanisms in corn roots and mitochondria. In mitochondria we find that only citrate and isocitrate are transported by the H/sup +//citrate symporter. However, the in vivo function of this carrier remains in doubt because citrate does not appear to be an effective substrate for corn mitochondria. Studies with roots have been directed to why various types of injury or shock all result in temporary blockage of the H/sup +/-efflux pump in the plasmamembrane. It appears this may be due to an injury-mediated Ca/sup 2 +/ influx into the tissue, which by raising free Ca/sup 2 +/ in the cytosal activates calmodulin (CaM). In turn, the Ca.CaM complex appears to activate protein kinase, phosphorylating membrane proteins. It is possible that one of these phosphorylated proteins is responsible for inactivation of the H/sup +/-ATPase. Future work is planned around the consequences of Ca/sup 2 +/ influx into the root cell subsequent to injury, investigating the recovery of the H/sup +/-ATPase and the initiation of the biosyntheses which lead to augmented ion transport.
- Research Organization:
- Illinois Univ., Urbana (USA). Dept. of Botany
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC02-76EV00790
- OSTI ID:
- 6623064
- Report Number(s):
- DOE/EV/00790-T1; ON: DE82021134
- Resource Relation:
- Other Information: Portions are illegible in microfiche products
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CELL MEMBRANES
BIOLOGICAL FUNCTIONS
MEMBRANE TRANSPORT
BIOLOGICAL MODELS
PLANTS
ATP-ASE
CALCIUM 45
CALCIUM COMPOUNDS
CATIONS
ROOT ABSORPTION
ABSORPTION
ACID ANHYDRASES
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METAL COMPOUNDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CALCIUM ISOTOPES
CELL CONSTITUENTS
CHARGED PARTICLES
DAYS LIVING RADIOISOTOPES
ENZYMES
EVEN-ODD NUCLEI
FUNCTIONS
HYDROLASES
INTERMEDIATE MASS NUCLEI
IONS
ISOTOPES
MEMBRANES
NUCLEI
PHOSPHOHYDROLASES
RADIOISOTOPES
UPTAKE
550201* - Biochemistry- Tracer Techniques