In vitro hydrogen production by glucose dehydrogenase and hydrogenase

Conference · Tue Oct 01 00:00:00 EDT 1996

DOI:https://doi.org/10.1038/nbt0796-872· OSTI ID:447146

Woodward, J ^[1]

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1. Oak Ridge National Lab., TN (United States)

A new in vitro enzymatic pathway for the generation of molecular hydrogen from glucose has been demonstrated. The reaction is based upon the oxidation of glucose by Thermoplasma acidophilum glucose dehydrogenase with the concomitant oxidation of NADPH by Pyrococcus furiosus hydrogenase. Stoichiometric yields of hydrogen were produced from glucose with continuous cofactor recycle. This simple system may provide a method for the biological production of hydrogen from renewable sources. In addition, the other product of this reaction, gluconic acid, is a high-value commodity chemical.

• Research Organization: National Renewable Energy Lab. (NREL), Golden, CO (United States)
• DOE Contract Number: AC05-96OR22464
• OSTI ID: 447146
• Report Number(s): NREL/CP-430-21968-Vol.1; CONF-9605195-Vol.1; ON: DE97000053; TRN: 97:001172-0014
• Resource Relation: Journal Volume: 14; Journal Issue: 7; Conference: 1996 annual hydrogen peer review for DOE, Miami, FL (United States), 1-3 May 1996; Other Information: PBD: Oct 1996; Related Information: Is Part Of Proceedings of the 1996 US DOE hydrogen program review. Volume 1; PB: 575 p.
• Country of Publication: United States
• Language: English

Related Subjects

08 HYDROGEN FUEL
GLUCOSE
OXIDATION
HYDROGEN PRODUCTION
ENZYME ACTIVITY
IN VITRO
BIOMASS