Mechanism of the cadherin–catenin F-actin catch bond interaction

Wang, Amy; Dunn, Alexander R.; Weis, William I.

doi:10.7554/elife.80130

Title: Mechanism of the cadherin–catenin F-actin catch bond interaction

Journal Article · Mon Aug 01 00:00:00 EDT 2022 · eLife

DOI:https://doi.org/10.7554/elife.80130· OSTI ID:1983349

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Stanford Univ., CA (United States)

Mechanotransduction at cell–cell adhesions is crucial for the structural integrity, organization, and morphogenesis of epithelia. At cell–cell junctions, ternary E-cadherin/β-catenin/αE-catenin complexes sense and transmit mechanical load by binding to F-actin. The interaction with F-actin, described as a two-state catch bond, is weak in solution but is strengthened by applied force due to force-dependent transitions between weak and strong actin-binding states. Here, we provide direct evidence from optical trapping experiments that the catch bond property principally resides in the αE-catenin actin-binding domain (ABD). Consistent with our previously proposed model, the deletion of the first helix of the five-helix ABD bundle enables stable interactions with F-actin under minimal load that are well described by a single-state slip bond, even when αE-catenin is complexed with β-catenin and E-cadherin. Our data argue for a conserved catch bond mechanism for adhesion proteins with structurally similar ABDs. We also demonstrate that a stably bound ABD strengthens load-dependent binding interactions between a neighboring complex and F-actin, but the presence of the other αE-catenin domains weakens this effect. These results provide mechanistic insight to the cooperative binding of the cadherin–catenin complex to F-actin, which regulate dynamic cytoskeletal linkages in epithelial tissues.

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Research Organization:: SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States). Stanford Synchrotron Radiation Lightsource (SSRL)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); National Institutes of Health (NIH); National Science Foundation (NSF)

Grant/Contract Number:: AC02-76SF00515; R01GM114462; R35GM130332; R35GM131747; T32GM120007; P30GM133894

OSTI ID:: 1983349

Journal Information:: eLife, Vol. 11; ISSN 2050-084X

Publisher:: eLife Sciences Publications, Ltd.Copyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
structural biology
molecular biophysics
cell adhesion
α-catenin
β-catenin
actin
cadherin
optical trap

Title: Mechanism of the cadherin–catenin F-actin catch bond interaction

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