Dynamics of Streptococcus mutans Transcriptome in Response to Starch and Sucrose during Biofilm Development

Journal Article · Tue Oct 19 00:00:00 EDT 2010 · PLoS ONE

DOI:https://doi.org/10.1371/journal.pone.0013478· OSTI ID:1627430

Klein, Marlise I. ^[1]; DeBaz, Lena ^[1]; Agidi, Senyo ^[1]; Lee, Herbert ^[2]; Xie, Gary ^[2]; Lin, Amy H. -M. ^[3]; Hamaker, Bruce R. ^[3]; Lemos, José A. ^[1]; Koo, Hyun ^[1]

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1. University of Rochester, NY (United States)
2. Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
3. Purdue University, West Lafayette, IN (United States)

The combination of sucrose and starch in the presence of surface-adsorbed salivary a-amylase and bacterial glucosyltransferases increase the formation of a structurally and metabolically distinctive biofilm by Streptococcus mutans. This host-pathogen-diet interaction may modulate the formation of pathogenic biofilms related to dental caries disease. We conducted a comprehensive study to further investigate the influence of the dietary carbohydrates on S. mutanstranscriptome at distinct stages of biofilm development using whole genomic profiling with a new computational tool (MDV) for data mining. S. mutans UA159 biofilms were formed on amylase-active saliva coated hydroxyapatite discs in the presence of various concentrations of sucrose alone (ranging from 0.25 to 5% w/v) or in combination with starch (0.5 to 1% w/v). Overall, the presence of sucrose and starch (suc+st) influenced the dynamics of S. mutans transcriptome (vs. sucrose alone), which may be associated with gradual digestion of starch by surface-adsorbed amylase. At 21 h of biofilm formation, most of the differentially expressed genes were related to sugar metabolism, such as upregulation of genes involved in maltose/maltotriose uptake and glycogen synthesis. In addition, the groEL/groES chaperones were induced in the suc+stbiofilm, indicating that presence of starch hydrolysates may cause environmental stress. In contrast, at 30 h of biofilm development, multiple genes associated with sugar uptake/transport (e.g. maltose), two-component systems, fermentation/ glycolysis and iron transport were differentially expressed in suc+st-biofilms (vs. sucrose-biofilms). Interestingly, lytT (bacteria autolysis) was upregulated, which was correlated with presence of extracellular DNA in the matrix of suc+stbiofilms. Specific genes related to carbohydrate uptake and glycogen metabolism were detected in suc+st-biofilms in more than one time point, indicating an association between presence of starch hydrolysates and intracellular polysaccharide storage. Our data show complex remodeling of S. mutans-transcriptome in response to changing environmental conditions in situ, which could modulate the dynamics of biofilm development and pathogenicity.

• Research Organization: Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
• Sponsoring Organization: USDOE Office of Science (SC), Biological and Environmental Research (BER); National Institutes of Health (NIH); University of California
• Grant/Contract Number: AC52-06NA25396; Y1-DE-6006-02
• OSTI ID: 1627430
• Journal Information: PLoS ONE, Vol. 5, Issue 10; ISSN 1932-6203
• Publisher: Public Library of Science
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
starches
sucrose
bacterial biofilms
streptococcus mutans
DNA-binding proteins
biofilms
microarrays
gene expression