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Title: Life in Hot Carbon Monoxide: The Complete Genome Sequence of Carboxydothermus hydrogenoformans Z-2901

Journal Article · · PLoS Genetics
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  1. The Inst. for Genomic Research, Rockville, MD (United States)
  2. Georgia Inst. of Technology, Atlanta, GA (United States). School of Biology. Center for Bioinformatics and Computational Biology
  3. Univ. of Maryland Baltimore County (UMBC), Baltimore, MD (United States). Biotechnology Inst. Center of Marine Biotechnology
  4. The Inst. for Genomic Research, Rockville, MD (United States); Johns Hopkins Univ., Baltimore, MD (United States)
+ Show Author Affiliations

We report here the sequencing and analysis of the genome of the thermophilic bacterium Carboxydothermus hydrogenoformans Z-2901. This species is a model for studies of hydrogenogens, which are diverse bacteria and archaea that grow anaerobically utilizing carbon monoxide (CO) as their sole carbon source and water as an electron acceptor, producing carbon dioxide and hydrogen as waste products. Organisms that make use of CO do so through carbon monoxide dehydrogenase complexes. Remarkably, analysis of the genome of C. hydrogenoformans reveals the presence of at least five highly differentiated anaerobic carbon monoxide dehydrogenase complexes, which may in part explain how this species is able to grow so much more rapidly on CO than many other species. Analysis of the genome also has provided many general insights into the metabolism of this organism which should make it easier to use it as a source of biologically produced hydrogen gas. One surprising finding is the presence of many genes previously found only in sporulating species in the Firmicutes Phylum. Although this species is also a Firmicutes, it was not known to sporulate previously. Here we show that it does sporulate and because it is missing many of the genes involved in sporulation in other species, this organism may serve as a ‘‘minimal’’ model for sporulation studies. In addition, using phylogenetic profile analysis, we have identified many uncharacterized gene families found in all known sporulating Firmicutes, but not in any non-sporulating bacteria, including a sigma factor not known to be involved in sporulation previously.

Research Organization:
The Inst. for Genomic Research, Rockville, MD (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division; National Science Foundation (NSF); National Institutes of Health (NIH)
Grant/Contract Number:
FC02-95ER61962; MCB-02383387; GM072285
OSTI ID:
1627264
Journal Information:
PLoS Genetics, Vol. 1, Issue 5; ISSN 1553-7404
Publisher:
Public Library of ScienceCopyright Statement
Country of Publication:
United States
Language:
English

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