Hypochromicity in red blood cells: an experimental and theoretical investigation
- University of South Florida, Tampa, FL (United States)
- Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
- Claro Scientific, St. Petersburg, FL (United States); Florida Blood Services, St. Petersburg, FL (United States)
- Florida Blood Services, St. Petersburg, FL (United States)
Multiwavelength UV-visible transmission spectrophotometry is a useful tool for the examination of micron-size particle suspensions in the context of particle size and chemical composition. This paper reports the reliability of this method to characterize the spectra of purified red blood cells both in their physiological state and with modified hemoglobin content. Previous studies have suggested the contribution of hypochromism on the particle spectra caused by the close electronic interaction of the encapsulated chromophores. Our research shows, however, that this perceived hypochromism can be accounted for by considering two important issues: the acceptance angle of the instrument and the combined scattering and absorption effect of light on the particles. In order to establish these ideas, spectral analysis was performed on purified and modified red cells where the latter was accomplished with a modified hypotonic shock protocol that altered the hemoglobin concentration within the cells. Moreover, the Mie theory was used to successfully simulate the spectral features and trends of the red cells. With this combination of experimental and theoretical exploration, definition of hypochromism has been extended to two subcategories.
- Research Organization:
- Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
- Sponsoring Organization:
- USDOE Office of Science (SC), Biological and Environmental Research (BER); National Science Foundation (NSF)
- Grant/Contract Number:
- AC52-06NA25396; W-7406-ENG-36; EEC-94-02989
- OSTI ID:
- 1627120
- Journal Information:
- Biomedical Optics Express, Vol. 2, Issue 8; ISSN 2156-7085
- Publisher:
- Optical Society of America (OSA)Copyright Statement
- Country of Publication:
- United States
- Language:
- English
Refractive index of solutions of human hemoglobin from the near-infrared to the ultraviolet range: Kramers-Kronig analysis
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journal | November 2012 |
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