Quantitative trait locus mapping of Populus bark features and stem diameter

Bdeir, Roba; Muchero, Wellington; Yordanov, Yordan; Tuskan, Gerald A.; Busov, Victor; Gailing, Oliver

doi:10.1186/s12870-017-1166-4

Title: Quantitative trait locus mapping of Populus bark features and stem diameter

Journal Article · Tue Nov 28 00:00:00 EST 2017 · BMC Plant Biology

DOI:https://doi.org/10.1186/s12870-017-1166-4· OSTI ID:1626848

Bdeir, Roba ^[1]; Muchero, Wellington ^[2]; Yordanov, Yordan ^[3]; Tuskan, Gerald A. ^[2]; Busov, Victor ^[1]; Gailing, Oliver ^[1]

Michigan Technological Univ., Houghton, MI (United States). School of Forest Resources and Environmental Science
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Div.
Eastern Illinois Univ., Charleston, IL (United States). Dept. of Biology

Background: Bark plays important roles in photosynthate transport and storage, along with physical and chemical protection. Bark texture varies extensively among species, from smooth to fissured to deeply furrowed, but its genetic control is unknown. This study sought to determine the main genomic regions associated with natural variation in bark features and stem diameter. Quantitative trait loci (QTL) were mapped using an interspecific pseudo-backcross pedigree (Populus trichocarpa x P. deltoides and P. deltoides) for bark texture, bark thickness and diameter collected across three years, two sites and three biological replicates per site. Results: QTL specific to bark texture were highly reproducible in shared intervals across sites, years and replicates. Significant positive correlations and co-localization between trait QTL suggest pleiotropic regulators or closely linked genes. A list of candidate genes with related putative function, location close to QTL maxima and with the highest expression level in the phloem, xylem and cambium was identified. Conclusion: Candidate genes for bark texture included an ortholog of Arabidopsis ANAC104 (PopNAC128), which plays a role in lignified fiber cell and ray development, as well as Pinin and Fasciclin (PopFLA) genes with a role in cell adhesion, cell shape and migration. The results presented in this study provide a basis for future genomic characterization of genes found within the QTL for bark texture, bark thickness and diameter in order to better understand stem and bark development in Populus and other woody perennial plants. The QTL mapping approach identified a list of prime candidate genes for further validation using functional genomics or forward genetics approaches.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); USDA; National Science Foundation (NSF)

Grant/Contract Number:: AC05-00OR22725; 2012–67,013-19,389; 1230803

OSTI ID:: 1626848

Journal Information:: BMC Plant Biology, Vol. 17, Issue 1; ISSN 1471-2229

Publisher:: BioMed CentralCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 12 works

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Additional file 1: Figure S1. of Quantitative trait locus mapping of Populus bark features and stem diameter Bdeir, Roba; Muchero, Wellington; Yordanov, Yordan https://doi.org/10.6084/M9.FIGSHARE.5639785 The current record is supplemented by this image	image	January 2017
Additional file 1: Figure S1. of Quantitative trait locus mapping of Populus bark features and stem diameter Bdeir, Roba; Muchero, Wellington; Yordanov, Yordan https://doi.org/10.6084/m9.figshare.5639785.v1 The current record is supplemented by this image	image	January 2017
Additional file 2: Table S1. of Quantitative trait locus mapping of Populus bark features and stem diameter Bdeir, Roba; Muchero, Wellington; Yordanov, Yordan https://doi.org/10.6084/M9.FIGSHARE.5639806 The current record is supplemented by this dataset	dataset	January 2017
Additional file 2: Table S1. of Quantitative trait locus mapping of Populus bark features and stem diameter Bdeir, Roba; Muchero, Wellington; Yordanov, Yordan https://doi.org/10.6084/m9.figshare.5639806.v1 The current record is supplemented by this dataset	dataset	January 2017
Additional file 3: FigureS2. of Quantitative trait locus mapping of Populus bark features and stem diameter Bdeir, Roba; Muchero, Wellington; Yordanov, Yordan https://doi.org/10.6084/M9.FIGSHARE.5639833 The current record is supplemented by this image	image	January 2017
Additional file 3: FigureS2. of Quantitative trait locus mapping of Populus bark features and stem diameter Bdeir, Roba; Muchero, Wellington; Yordanov, Yordan https://doi.org/10.6084/m9.figshare.5639833.v1 The current record is supplemented by this image	image	January 2017
Additional file 4: Table S2. of Quantitative trait locus mapping of Populus bark features and stem diameter Bdeir, Roba; Muchero, Wellington; Yordanov, Yordan https://doi.org/10.6084/M9.FIGSHARE.5639848 The current record is supplemented by this dataset	dataset	January 2017
Additional file 4: Table S2. of Quantitative trait locus mapping of Populus bark features and stem diameter Bdeir, Roba; Muchero, Wellington; Yordanov, Yordan https://doi.org/10.6084/m9.figshare.5639848.v1 The current record is supplemented by this dataset	dataset	January 2017
Additional file 5: Table S3. of Quantitative trait locus mapping of Populus bark features and stem diameter Bdeir, Roba; Muchero, Wellington; Yordanov, Yordan https://doi.org/10.6084/m9.figshare.5639872 The current record is supplemented by this text	text	January 2017
Additional file 5: Table S3. of Quantitative trait locus mapping of Populus bark features and stem diameter Bdeir, Roba; Muchero, Wellington; Yordanov, Yordan https://doi.org/10.6084/m9.figshare.5639872.v1 The current record is supplemented by this text	text	January 2017
Additional file 6: Table S4. of Quantitative trait locus mapping of Populus bark features and stem diameter Bdeir, Roba; Muchero, Wellington; Yordanov, Yordan https://doi.org/10.6084/M9.FIGSHARE.5639884 The current record is supplemented by this dataset	dataset	January 2017
Additional file 6: Table S4. of Quantitative trait locus mapping of Populus bark features and stem diameter Bdeir, Roba; Muchero, Wellington; Yordanov, Yordan https://doi.org/10.6084/m9.figshare.5639884.v1 The current record is supplemented by this dataset	dataset	January 2017
Additional file 7: Table S5. of Quantitative trait locus mapping of Populus bark features and stem diameter Bdeir, Roba; Muchero, Wellington; Yordanov, Yordan https://doi.org/10.6084/M9.FIGSHARE.5639902 The current record is supplemented by this dataset	dataset	January 2017
Additional file 7: Table S5. of Quantitative trait locus mapping of Populus bark features and stem diameter Bdeir, Roba; Muchero, Wellington; Yordanov, Yordan https://doi.org/10.6084/m9.figshare.5639902.v1 The current record is supplemented by this dataset	dataset	January 2017
Quantitative trait locus mapping of Populus bark features and stem diameter Bdeir, Roba; Muchero, Wellington; Yordanov, Yordan https://doi.org/10.6084/M9.FIGSHARE.C.3940078 The current record is supplemented by this collection	collection	January 2017
Quantitative trait locus mapping of Populus bark features and stem diameter Bdeir, Roba; Muchero, Wellington; Yordanov, Yordan https://doi.org/10.6084/m9.figshare.c.3940078.v1 The current record is supplemented by this collection	collection	January 2017