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Title: Deep phylogenomics of a tandem-repeat galectin regulating appendicular skeletal pattern formation

Journal Article · · BMC Evolutionary Biology (Online)
 [1];  [2];  [3];  [4];  [5]
  1. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Life Sciences Div.; Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bangalore, 560012, India
  2. Univ. of California, Irvine, CA (United States). Dept. of Ecology and Evolutionary Biology
  3. Western Washington Univ., Bellingham, WA (United States). Dept. of Mathematics
  4. Yale Univ., New Haven, CT, (United States). Dept. of Ecology and Evolutionary Biology; Univ. of Minnesota, Minneapolis, MN, (United States). Minnesota Center for Philosophy of Science
  5. New York Medical College, Valhalla, NY (United States). Dept. of Cell Biology and Anatomy
+ Show Author Affiliations

Background A multiscale network of two galectins Galectin-1 (Gal-1) and Galectin-8 (Gal-8) patterns the avian limb skeleton. Among vertebrates with paired appendages, chondrichthyan fins typically have one or more cartilage plates and many repeating parallel endoskeletal elements, actinopterygian fins have more varied patterns of nodules, bars and plates, while tetrapod limbs exhibit tandem arrays of few, proximodistally increasing numbers of elements. We applied a comparative genomic and protein evolution approach to understand the origin of the galectin patterning network. Having previously observed a phylogenetic constraint on Gal-1 structure across vertebrates, we asked whether evolutionary changes of Gal-8 could have critically contributed to the origin of the tetrapod pattern. Results Translocations, duplications, and losses of Gal-8 genes in Actinopterygii established them in different genomic locations from those that the Sarcopterygii (including the tetrapods) share with chondrichthyans. The sarcopterygian Gal-8 genes acquired a potentially regulatory non-coding motif and underwent purifying selection. The actinopterygian Gal-8 genes, in contrast, did not acquire the non-coding motif and underwent positive selection. Conclusion These observations interpreted through the lens of a reaction-diffusion-adhesion model based on avian experimental findings can account for the distinct endoskeletal patterns of cartilaginous, ray-finned, and lobe-finned fishes, and the stereotypical limb skeletons of tetrapods.

Research Organization:
Lawrence Berkeley National Lab (LBNL), Berkeley, CA (United States)
Sponsoring Organization:
USDOE Office of Science (SC)
Grant/Contract Number:
AC02-05CH11231
OSTI ID:
1626778
Journal Information:
BMC Evolutionary Biology (Online), Vol. 16, Issue 1; ISSN 1471-2148
Publisher:
BioMed CentralCopyright Statement
Country of Publication:
United States
Language:
English

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Cited By (2)

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Data from: Deep phylogenomics of a tandem-repeat galectin regulating appendicular skeletal pattern formation dataset January 2016

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