Engineering Clostridium for improved solvent production: recent progress and perspective

Cheng, Chi; Bao, Teng; Yang, Shang-Tian

doi:10.1007/s00253-019-09916-7

Title: Engineering Clostridium for improved solvent production: recent progress and perspective

Journal Article · Wed May 29 00:00:00 EDT 2019 · Applied Microbiology and Biotechnology

DOI:https://doi.org/10.1007/s00253-019-09916-7· OSTI ID:1572190

Cheng, Chi ^[1]; Bao, Teng ^[2];

^[2]

Dalian Univ. of Technology, Dalian (China); The Ohio State Univ., Columbus, OH (United States)
The Ohio State Univ., Columbus, OH (United States)

Clostridia are Gram-positive, spore-forming, obligate anaerobic bacteria that can produce solvents such as acetone, ethanol, and butanol, which can be used as biofuels or building-block chemicals. Many successful attempts have been made to improve solvent yield and titer from sugars through metabolic engineering of solventogenic and acidogenic clostridia. More recently, cellulolytic and acetogenic clostridia have also attracted high interests for their ability to utilize low-cost renewable substrates such as cellulose and syngas. Process engineering such as in situ butanol recovery and consolidated bioprocessing (CBP) has been developed for improved solvent titer and productivity. This review focuses on metabolic and process engineering strategies for solvent production from sugars, lignocellulosic biomass, and syngas by various clostridia, including conventional solventogenic Clostridium acetobutylicum, engineered acidogens such as C. tyrobutyricum and C. cellulovorans, and carboxydotrophic acetogens such as C. carboxidivorans and C. ljungdahlii.

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Research Organization:: The Ohio State Univ., Columbus, OH (United States)

Sponsoring Organization:: USDOE Office of Energy Efficiency and Renewable Energy (EERE), Sustainable Transportation Office. Bioenergy Technologies Office

Grant/Contract Number:: EE0007005

OSTI ID:: 1572190

Journal Information:: Applied Microbiology and Biotechnology, Vol. 103, Issue 14; ISSN 0175-7598

Publisher:: SpringerCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 49 works

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Control of solvent production by sigma‐54 factor and the transcriptional activator AdhR in Clostridium beijerinckii Yang, Bin; Nie, Xiaoqun; Gu, Yang Microbial Biotechnology, Vol. 13, Issue 2 https://doi.org/10.1111/1751-7915.13505	journal	August 2019
A microplate screen to estimate metal-binding affinities of metalloproteins Diep, Patrick; Mahadevan, Radhakrishnan; Yakunin, Alexander F. Analytical Biochemistry, Vol. 609 https://doi.org/10.1016/j.ab.2020.113836	journal	November 2020

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