Subsidized or stressed? Shifts in freshwater benthic microbial metagenomics along a gradient of alkaline coal mine drainage

Bier, Raven L.; Wernegreen, Jennifer J.; Vilgalys, Rytas J.; Ellis, Joseph Christopher; Bernhardt, Emily S.

doi:10.1002/lno.11301

Title: Subsidized or stressed? Shifts in freshwater benthic microbial metagenomics along a gradient of alkaline coal mine drainage

Journal Article · Thu Aug 15 00:00:00 EDT 2019 · Limnology and Oceanography

DOI:https://doi.org/10.1002/lno.11301· OSTI ID:1561663

^[1]; Wernegreen, Jennifer J. ^[2]; Vilgalys, Rytas J. ^[2];

^[3];

^[2]

Stroud Water Research Center, Avondale, PA (United States); Duke Univ., Durham, NC (United States)
Duke Univ., Durham, NC (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Chemical pollution mixtures enter aquatic environments and interact with microorganisms in eclectic ways with disparate consequences for microbial ecosystem services. Can using a thermodynamic framework help to determine the net influence of a chemical mixture on the functional capacity of benthic microbial communities? In this study, we examined this question by comparing benthic stream microbial communities exposed to a gradient of neutral-alkaline coalmine effluent. Using a combination of approaches (metagenomics, quantitative polymerase chain reaction [qPCR], and functional assays), we show that functional genes and pathways of microbial communities growing in mine effluent differed in composition, but not diversity. The majority of functional genes and pathways that changed decreased at sites exposed to mine effluent, resulting in lower abundances of nitrogenase and methanogen genes and fermentation pathways. However, selenate reductase gene abundance increased with water and sediment concentration of an ecologically important contaminant at mined sites: selenium. Denitrification genes nosZ and nirK differed between sites: metagenome-based nosZ increased with dissolved nitrate concentration and qPCR-based nirK had a hump-shaped pattern across the mining gradient. Osmoprotectant gene abundance did not change. Extracellular enzyme assays and alkaline phosphatase gene relative abundance suggested that mined stream microbial communities may be constrained by phosphorus bioavailability. Subsidies and stressors related to changes in a set of functional genes and pathways, but differences were not consistently predictable using thermodynamic expectations. Laslty, this suggests that pairing hypotheses for expected subsidies and stressors with post hoc explorations can yield valuable directions for future study of how microbial functional capacity responds to pollutant mixtures.

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Research Organization:: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1561663

Journal Information:: Limnology and Oceanography, Vol. 65, Issue S1; ISSN 0024-3590

Publisher:: Association for the Sciences of Limnology and Oceanography - WileyCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 5 works

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