Electrospray sample injection for single-particle imaging with x-ray lasers

Bielecki, Johan; Hantke, Max F.; Daurer, Benedikt J.; Reddy, Hemanth K. N.; Hasse, Dirk; Larsson, Daniel S. D.; Gunn, Laura H.; Svenda, Martin; Munke, Anna; Sellberg, Jonas A.; Flueckiger, Leonie; Pietrini, Alberto; Nettelblad, Carl; Lundholm, Ida; Carlsson, Gunilla; Okamoto, Kenta; Timneanu, Nicusor; Westphal, Daniel; Kulyk, Olena; Higashiura, Akifumi; van der Schot, Gijs; Loh, Ne-Te Duane; Wysong, Taylor E.; Bostedt, Christoph; Gorkhover, Tais; Iwan, Bianca; Seibert, M. Marvin; Osipov, Timur; Walter, Peter; Hart, Philip; Bucher, Maximilian; Ulmer, Anatoli; Ray, Dipanwita; Carini, Gabriella; Ferguson, Ken R.; Andersson, Inger; Andreasson, Jakob; Hajdu, Janos; Maia, Filipe R. N. C.

doi:10.1126/sciadv.aav8801

Title: Electrospray sample injection for single-particle imaging with x-ray lasers

Journal Article · Fri May 03 00:00:00 EDT 2019 · Science Advances

DOI:https://doi.org/10.1126/sciadv.aav8801· OSTI ID:1532400

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Uppsala Univ., Uppsala (Sweden); European XFEL GmbH, Schenefeld (Germany)
Uppsala Univ., Uppsala (Sweden); Oxford Univ., Oxford (United Kingdom); Refeyn Ltd., Oxford (United Kingdom)
Uppsala Univ., Uppsala (Sweden); National Univ. of Singapore (Singapore)
Uppsala Univ., Uppsala (Sweden)
Uppsala Univ., Uppsala (Sweden); KTH Royal Institute of Technology, Stockholm (Sweden)
La Trobe Univ., Melbourne, VIC (Australia)
Academy of Sciences of the Czech Republic, Prague (Czech Republic)
Osaka Univ., Osaka (Japan); Hiroshima Univ., Hiroshima (Japan)
Uppsala Univ., Uppsala (Sweden); Utrecht Univ., Utrecht (Netherlands)
National Univ. of Singapore (Singapore)
SLAC National Accelerator Lab., Menlo Park, CA (United States)
Argonne National Lab. (ANL), Argonne, IL (United States); Northwestern Univ., Evanston, IL (United States)
SLAC National Accelerator Lab., Menlo Park, CA (United States); Leibniz Univ. Hannover, Hannover (Germany)
Argonne National Lab. (ANL), Argonne, IL (United States)
Technische Univ. Berlin, Berlin (Germany)
SLAC National Accelerator Lab., Menlo Park, CA (United States); Brookhaven National Lab. (BNL), Upton, NY (United States)
Academy of Sciences of the Czech Republic, Prague (Czech Republic); Chalmers Univ. of Technology, Gothenburg (Sweden)
Uppsala Univ., Uppsala (Sweden); Academy of Sciences of the Czech Republic; Prague (Czech Republic)
Uppsala Univ., Uppsala (Sweden); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

The possibility of imaging single proteins constitutes an exciting challenge for x-ray lasers. Despite encouraging results on large particles, imaging small particles has proven to be difficult for two reasons: not quite high enough pulse intensity from currently available x-ray lasers and, as we demonstrate here, contamination of the aerosolized molecules by nonvolatile contaminants in the solution. The amount of contamination on the sample depends on the initial droplet size during aerosolization. Here, we show that, with our electrospray injector, we can decrease the size of aerosol droplets and demonstrate virtually contaminant-free sample delivery of organelles, small virions, and proteins. The results presented here, together with the increased performance of next-generation x-ray lasers, constitute an important stepping stone toward the ultimate goal of protein structure determination from imaging at room temperature and high temporal resolution.

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Research Organization:: SLAC National Accelerator Lab., Menlo Park, CA (United States); Argonne National Lab. (ANL), Argonne, IL (United States); Brookhaven National Lab. (BNL), Upton, NY (United States)

Sponsoring Organization:: Swedish Foundation for Strategic Research (SSF); USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences, and Biosciences Division; Swedish Research Council (SRC); Knut and Alice Wallenberg Foundation; European Research Council (ERC); European Regional Development Fund (ERDF); Wellcome Trust; Volkswagen Foundation

Grant/Contract Number:: AC02-76SF00515; AC02-06CH11357; SC0012704

OSTI ID:: 1532400

Alternate ID(s):: OSTI ID: 1559029; OSTI ID: 1571429

Report Number(s):: BNL-212202-2019-JAAM

Journal Information:: Science Advances, Vol. 5, Issue 5; ISSN 2375-2548

Publisher:: AAASCopyright Statement

Country of Publication:: United States

Language:: English

Citation Metrics:

Cited by: 33 works

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