Microfabricated multi-frequency particle impedance characterization system
We have developed a microfabricated flow-through impedance characterization system capable of performing AC, multi-frequency measurements on cells and other particles. The sensor measures both the resistive and reactive impedance of passing particles, at rates of up to 100 particles per second. Its operational bandwidth approaches 10 MHz with a signal-to-noise ratio of approximately 40 dB. Particle impedance is measured at three or more frequencies simultaneously, enabling the derivation of multiple particle parameters. This constitutes an improvement to the well-established technique of DC particle sizing via the Coulter Principle. Human peripheral blood granulocyte radius, membrane capacitance, and cytoplasmic conductivity were measured (r = 4.1 {micro}m, C{sub mem} = 0.9 {micro}F/cm{sup 2}, {sigma}{sub int} = 0.66 S/m) and were found to be consistent with published values.
- Research Organization:
- Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
- Sponsoring Organization:
- US Department of Energy (US)
- DOE Contract Number:
- W-7405-ENG-48
- OSTI ID:
- 15005423
- Report Number(s):
- UCRL-JC-136585; TRN: US200322%%431
- Resource Relation:
- Conference: Micro TAS 2000, Twente (NL), 05/14/2000--05/18/2000; Other Information: PBD: 1 Mar 2000
- Country of Publication:
- United States
- Language:
- English
Automated electrorotation: dielectric characterization of living cells by real-time motion estimation
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journal | March 1998 |
Electro-rotation: development of a technique for dielectric measurements on individual cells and particles
|
journal | September 1988 |
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