Zika virus produces noncoding RNAs using a multi-pseudoknot structure that confounds a cellular exonuclease

Akiyama, Benjamin M.; Laurence, Hannah M.; Massey, Aaron R.; Costantino, David A.; Xie, Xuping; Yang, Yujiao; Shi, Pei-Yong -Y.; Nix, Jay C.; Beckham, J. David; Kieft, Jeffrey S.

doi:10.1126/science.aah3963

Title: Zika virus produces noncoding RNAs using a multi-pseudoknot structure that confounds a cellular exonuclease

Journal Article · Thu Nov 10 00:00:00 EST 2016 · Science

DOI:https://doi.org/10.1126/science.aah3963· OSTI ID:1411558

Akiyama, Benjamin M. ^[1]; Laurence, Hannah M. ^[2]; Massey, Aaron R. ^[3]; Costantino, David A. ^[1]; Xie, Xuping ^[4]; Yang, Yujiao ^[4]; Shi, Pei-Yong -Y. ^[4]; Nix, Jay C. ^[5]; Beckham, J. David ^[3]; Kieft, Jeffrey S. ^[6]

Univ. of Colorado, Aurora, CO (United States). Denver School of Medicine, Dept. of Biochemistry and Molecular Genetics
Univ. of Colorado, Aurora, CO (United States). Denver School of Medicine, Dept. of Biochemistry and Molecular Genetics; Univ. of Colorado, Aurora, CO (United States). Denver School of Medicine, Howard Hughes Medical Inst. (HHMI); Univ. of California, Davis, CA (United States). School of Veterinary Medicine
Univ. of Colorado, Aurora, CO (United States). Denver School of Medicine, Dept. Medicine, Division of Infectious Diseases
Univ. of Texas, Galveston, TX (United States). Medical Branch, Dept. of Biochemistry and Molecular Biology
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Advanced Light Source (ALS), Molecular Biology Consortium
Univ. of Colorado, Aurora, CO (United States). Denver School of Medicine, Dept. of Biochemistry and Molecular Genetics; Univ. of Colorado, Aurora, CO (United States). Denver School of Medicine, RNA BioScience Initiative

The outbreak of Zika virus (ZIKV) and associated fetal microcephaly mandates efforts to understand the molecular processes of infection. Related flaviviruses produce noncoding subgenomic flaviviral RNAs (sfRNAs) that are linked to pathogenicity in fetal mice. These viruses make sfRNAs by co-opting a cellular exonuclease via structured RNAs called xrRNAs. We found that ZIKV-infected monkey and human epithelial cells, mouse neurons, and mosquito cells produce sfRNAs. The RNA structure that is responsible for ZIKV sfRNA production forms a complex fold that is likely found in many pathogenic flaviviruses. Mutations that disrupt the structure affect exonuclease resistance in vitro and sfRNA formation during infection. The complete ZIKV xrRNA structure clarifies the mechanism of exonuclease resistance and identifies features that may modulate function in diverse flaviviruses.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: AC02-05CH11231; R35GM118070; R01GM081346; P30CA046934; S10OD012033

OSTI ID:: 1411558

Journal Information:: Science, Vol. 354, Issue 6316; ISSN 0036-8075

Publisher:: AAASCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 158 works

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