A robust gene-stacking method utilizing yeast assembly for plant synthetic biology

Shih, Patrick M.; Vuu, Khanh; Mansoori, Nasim; Ayad, Leïla; Louie, Katherine B.; Bowen, Benjamin P.; Northen, Trent R.; Loqué, Dominique

doi:10.1038/ncomms13215

Title: A robust gene-stacking method utilizing yeast assembly for plant synthetic biology

Journal Article · Wed Oct 26 00:00:00 EDT 2016 · Nature Communications

DOI:https://doi.org/10.1038/ncomms13215· OSTI ID:1377550

Shih, Patrick M. ^[1]; Vuu, Khanh ^[1]; Mansoori, Nasim ^[1]; Ayad, Leïla ^[1]; Louie, Katherine B. ^[2]; Bowen, Benjamin P. ^[2]; Northen, Trent R. ^[3]; Loqué, Dominique ^[4]

Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States); USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Univ. Claude Bernard and CNRS, Lyon (France)

The advent and growth of synthetic biology has demonstrated its potential as a promising avenue of research to address many societal needs. But, plant synthetic biology efforts have been hampered by a dearth of DNA part libraries, versatile transformation vectors and efficient assembly strategies. We describe a versatile system (named jStack) utilizing yeast homologous recombination to efficiently assemble DNA into plant transformation vectors. We also demonstrate how this method can facilitate pathway engineering of molecules of pharmaceutical interest, production of potential biofuels and shuffling of disease-resistance traits between crop species. Our approach provides a powerful alternative to conventional strategies for stacking genes and traits to address many impending environmental and agricultural challenges.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1377550

Journal Information:: Nature Communications, Vol. 7; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 37 works

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