Crystallographic capture of a radical S-adenosylmethionine enzyme in the act of modifying tRNA

Schwalm, Erica L.; Grove, Tyler L.; Booker, Squire J.; Boal, Amie K.

doi:10.1126/science.aad5367

Title: Crystallographic capture of a radical S-adenosylmethionine enzyme in the act of modifying tRNA

Journal Article · Thu Apr 14 00:00:00 EDT 2016 · Science

DOI:https://doi.org/10.1126/science.aad5367· OSTI ID:1252748

Schwalm, Erica L. ^[1]; Grove, Tyler L. ^[1]; Booker, Squire J. ^[1]; Boal, Amie K. ^[1]

Pennsylvania State Univ., University Park, PA (United States)

RlmN is a dual-specificity RNA methylase that modifies C2 of adenosine 2503 (A2503) in 23S rRNA and C2 of adenosine 37 (A37) in several Escherichia coli transfer RNAs (tRNAs). A related methylase, Cfr, modifies C8 of A2503 via a similar mechanism, conferring resistance to multiple classes of antibiotics. Here, we report the x-ray structure of a key intermediate in the RlmN reaction, in which a Cys₁₁₈→Ala variant of the protein is cross-linked to a tRNA^Glu substrate through the terminal methylene carbon of a formerly methylcysteinyl residue and C2 of A37. RlmN contacts the entire length of tRNA^Glu, accessing A37 by using an induced-fit strategy that completely unfolds the tRNA anticodon stem-loop, which is likely critical for recognition of both tRNA and ribosomal RNA substrates.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 1252748

Journal Information:: Science, Vol. 352, Issue 6283; ISSN 0036-8075

Publisher:: AAASCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

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Cited by: 45 works

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