A novel inert crystal delivery medium for serial femtosecond crystallography

Conrad, Chelsie E.; Basu, Shibom; James, Daniel; Wang, Dingjie; Schaffer, Alexander; Roy-Chowdhury, Shatabdi; Zatsepin, Nadia A.; Aquila, Andrew; Coe, Jesse; Gati, Cornelius; Hunter, Mark S.; Koglin, Jason E.; Kupitz, Christopher; Nelson, Garrett; Subramanian, Ganesh; White, Thomas A.; Zhao, Yun; Zook, James; Boutet, Sébastien; Cherezov, Vadim; Spence, John C. H.; Fromme, Raimund; Weierstall, Uwe; Fromme, Petra

doi:10.1107/S2052252515009811

Title: A novel inert crystal delivery medium for serial femtosecond crystallography

Journal Article · Tue Jun 30 00:00:00 EDT 2015 · IUCrJ

DOI:https://doi.org/10.1107/S2052252515009811· OSTI ID:1208832

Conrad, Chelsie E. ^[1]; Basu, Shibom ^[2]; James, Daniel ^[2]; Wang, Dingjie ^[3]; Schaffer, Alexander ^[2]; Roy-Chowdhury, Shatabdi ^[2]; Zatsepin, Nadia A. ^[2]; Aquila, Andrew ^[4]; Coe, Jesse ^[2]; Gati, Cornelius ^[5]; Hunter, Mark S. ^[4]; Koglin, Jason E. ^[6]; Kupitz, Christopher ^[7]; Nelson, Garrett ^[2]; Subramanian, Ganesh ^[2]; White, Thomas A. ^[5]; Zhao, Yun ^[5]; Zook, James ^[2]; Boutet, Sébastien ^[6]; Cherezov, Vadim ^[8] more »

Arizona State Univ., Tempe, AZ (United States);The Biodesign Inst., Tempe, AZ (United States)
Arizona State Univ., Tempe, AZ (United States); The Biodesign Inst., Tempe, AZ (United States)
Arizona State Univ., Tempe, AZ (United States)
SLAC National Accelerator Laboratory, Menlo Park, CA (United States)
Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany)
SLAC National Accelerator Lab., Menlo Park, CA (United States)
Univ. of Wisconsin-Milwaukee, Milwaukee, WI (United States); The Biodesign Inst., Tempe, AZ (United States)
Univ. of Southern California, Los Angeles, CA (United States)

Serial femtosecond crystallography (SFX) has opened a new era in crystallography by permitting nearly damage-free, room-temperature structure determination of challenging proteins such as membrane proteins. In SFX, femtosecond X-ray free-electron laser pulses produce diffraction snapshots from nanocrystals and microcrystals delivered in a liquid jet, which leads to high protein consumption. A slow-moving stream of agarose has been developed as a new crystal delivery medium for SFX. It has low background scattering, is compatible with both soluble and membrane proteins, and can deliver the protein crystals at a wide range of temperatures down to 4°C. Using this crystal-laden agarose stream, the structure of a multi-subunit complex, phycocyanin, was solved to 2.5 Å resolution using 300 µg of microcrystals embedded into the agarose medium post-crystallization. The agarose delivery method reduces protein consumption by at least 100-fold and has the potential to be used for a diverse population of proteins, including membrane protein complexes.

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Research Organization:: SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: AC03-76SF00515; SC0010575

OSTI ID:: 1208832

Journal Information:: IUCrJ, Vol. 2, Issue 4; ISSN 2052-2525

Publisher:: International Union of CrystallographyCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 104 works

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59 BASIC BIOLOGICAL SCIENCES
serial femtosecond crystallography
viscous crystal delivery
protein complexes
membrane proteins
femtosecond studies
nanocrystals
coherent X-ray diffractive imaging
free-electron laser

Title: A novel inert crystal delivery medium for serial femtosecond crystallography

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