Observing heme doming in myoglobin with femtosecond X-ray absorption spectroscopy

Levantino, M.; Lemke, H. T.; Schirò, G.; Glownia, M.; Cupane, A.; Cammarata, M.

doi:10.1063/1.4921907

Title: Observing heme doming in myoglobin with femtosecond X-ray absorption spectroscopy

Journal Article · Wed Jul 01 00:00:00 EDT 2015 · Structural Dynamics

DOI:https://doi.org/10.1063/1.4921907· OSTI ID:1190858

Levantino, M. ^[1]; Lemke, H. T. ^[2]; Schirò, G. ^[3]; Glownia, M. ^[2]; Cupane, A. ^[1]; Cammarata, M. ^[4]

Univ. of Palermo, Palermo (Italy)
SLAC National Accelerator Lab., Menlo Park, CA (United States)
CNRS - Institut de Biologie Structurale, Grenoble 38044, France
Univ. of Rennes, Rennes (France)

We report time-resolved X-ray absorption measurements after photolysis of carbonmonoxy myoglobin performed at the LCLS X-ray free electron laser with nearly 100 fs (FWHM) time resolution. Data at the Fe K-edge reveal that the photoinduced structural changes at the heme occur in two steps, with a faster (~70 fs) relaxation preceding a slower (~400 fs) one. We tentatively attribute the first relaxation to a structural rearrangement induced by photolysis involving essentially only the heme chromophore and the second relaxation to a residual Fe motion out of the heme plane that is coupled to the displacement of myoglobin F-helix.

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Research Organization:: SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

OSTI ID:: 1190858

Journal Information:: Structural Dynamics, Vol. 2, Issue 4; ISSN 2329-7778

Publisher:: American Crystallographic Association/AIPCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 36 works

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