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Title: Compositions of orthogonal lysyl-tRNA and aminoacyl-tRNA synthetase pairs and uses thereof

Patent ·
OSTI ID:1126706
; ; ;

Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal lysyl-tRNAs, orthogonal lysyl-aminoacyl-tRNA synthetases, and orthogonal pairs of lysyl-tRNAs/synthetases, which incorporate homoglutamines into proteins are provided in response to a four base codon. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins with homoglutamines using these orthogonal pairs.

Research Organization:
The Scripps Research Institute, La Jolla, CA, USA
Sponsoring Organization:
USDOE
DOE Contract Number:
ER45812
Assignee:
The Scripps Research Institute (La Jolla, CA)
Patent Number(s):
8,669,073
Application Number:
13/246,490
OSTI ID:
1126706
Country of Publication:
United States
Language:
English

References (107)

Glycoprotein synthesis patent August 2005
In vivo incorporation of unnatural amino acids patent May 2006
Methods and compositions for the production of orthogonal tRNA-aminoacyl tRNA synthetase pairs patent August 2006
Glycoprotein synthesis patent October 2006
Glycoprotein synthesis patent February 2007
Glycoprotein synthesis patent April 2007
Glycoprotein synthesis patent May 2007
Glycoprotein synthesis patent July 2007
Glycoprotein synthesis patent August 2007
Methods and composition for the production of orthogonal tRNA-aminoacyltRNA synthetase pairs patent April 2008
In vivo incorporation of unnatural amino acids patent May 2008
In vivo site-specific incorporation of N-acetyl-galactosamine amino acids in eubacteria patent May 2008
Site specific incorporation of heavy atom-containing unnatural amino acids into proteins for structure determination patent July 2008
Site specific incorporation of keto amino acids into proteins patent October 2008
Site-specific incorporation of redox active amino acids into proteins patent February 2009
Site specific incorporation of keto amino acids into proteins patent April 2009
Compositions of orthogonal glutamyl-tRNA and aminoacyl-tRNA synthetase pairs and uses thereof patent May 2009
Glycoprotein synthesis patent July 2009
Compositions of orthogonal lysyl-tRNA and aminoacyl-tRNA synthetase pairs and uses thereof patent August 2009
Expanding the eukaryotic genetic code patent October 2009
Compositions of orthogonal lysyl-tRNA and aminoacyl-tRNA synthetase pairs and uses thereof patent December 2009
In vivo incorporation of unnatural amino acids patent December 2009
Protein arrays patent January 2010
Unnatural reactive amino acid genetic code additions patent-application December 2004
Expanding the eukaryotic genetic code patent-application January 2005
Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells patent-application June 2005
Methods and composition for the production of orthogonal tRNA-aminoacyltRNA synthetase pairs patent-application September 2005
In vivo incorporation of unnatural amino acids patent-application November 2005
Methods and composition for the production of orthogonal tRNA-aminoacyltRNA synthetase pairs patent-application March 2006
In vivo incorporation of alkynyl amino acids into proteins in eubacteria patent-application April 2006
Site-specific labeling of proteins for NMR studies patent-application May 2006
In vivo site-specific incorporation of N-acetyl-galactosamine amino acids in eubacteria patent-application May 2006
Adding photoregulated amino acids to the genetic code patent-application June 2006
Compositions of orthogonal leucyl-trna and aminoacyl-trna synthetase pairs and uses thereof patent-application July 2006
Unnatural reactive amino acid genetic code additions patent-application November 2006
Site-specific incorporation of redox active amino acids into proteins patent-application January 2007
Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells patent-application May 2007
Expanding the eukaryotic genetic code patent-application July 2007
Glycoprotein synthesis patent-application July 2007
Selective posttranslational modification of phage-displayed polypeptides patent-application August 2007
Genetically programmed expression of proteins containing the unnatural amino acid phenylselenocysteine patent-application October 2007
Systems for the expression of orthogonal translation components in eubacterial host cells patent-application December 2007
Genetically encoded fluorescent coumarin amino acids patent-application February 2008
In Vivo Site-Specific Incorporation of N-Acetyl-Galactosamine Amino Acids in Eubacteria patent-application March 2008
Genetically programmed expression of selectively sulfated proteins in eubacteria patent-application May 2008
Genetic incorporation of unnatural amino acids into proteins in mammalian cells patent-application July 2008
In vivo incorporation of unnatural amino acids patent-application July 2008
In vivo incorporation of unnatural amino acids patent-application July 2008
Site-Specific Labeling of Proteins for Nmr Studies patent-application July 2008
Expanding the eukaryotic genetic code patent-application July 2008
Glycoprotein synthesis patent-application September 2008
In Vivo Incorporation of Alkynyl Amino Acids into Proteins in Eubacteria patent-application September 2008
In vivo incorporation of unnatural amino acids patent-application September 2008
Site specific incorporation of keto amino acids into proteins patent-application September 2008
Expanding the eukaryotic genetic code patent-application September 2008
Orthogonal Translation Components for the in Vivo Incorporation of Unnatural Amino Acids patent-application September 2008
In vivo site-sepecific incorporation of N-acetyl-galactosamine amino acids in eubacteria patent-application October 2008
In vivo incorporation of unnatural amino acids patent-application October 2008
Methods and composition for the production of orthogonal tRNA-aminoacyl tRNA synthetase pairs patent-application February 2009
In vivo incorporation of unnatural amino acids patent-application March 2009
Site-specific labeling of proteins for NMR studies patent-application March 2009
Selective Posttranslational Modification of Phage-Displayed Polypeptides patent-application May 2009
Genetically Encoded Boronate Amino Acid patent-application June 2009
Directed evolution using proteins comprising unnatural amino acids patent-application June 2009
Systems for the Expression of Orthogonal Translation Components Eubacterial Host Cells patent-application July 2009
Adding photoregulated amino acids to the genetic code patent-application July 2009
In vivo unnatural amino acid expression in the methylotrophic yeast pichia pastoris patent-application August 2009
Genetic Incorporation of 3-Aminotyrosine Into Reductases patent-application August 2009
Genetically Programmed Expression of Proteins patent-application August 2009
Site specific incorporation of keto amino acids into proteins patent-application August 2009
Methods and compositions for the production of orthogonal tRNA-aminoacyl tRNA synthetase pairs patent-application September 2009
Breaking immunological toterance with a genetically encoded unnatural amino acid patent-application October 2009
Genetically programmed expression of selectively sulfated proteins in eubacteria patent-application November 2009
Genetically encoded coumarin amino acids patent-application December 2009
Site-specific incorporation of redox active amino acids into proteins patent-application December 2009
Glycoprotein synthesis patent-application January 2010
Genetic incorporation of unnatural amino acids into proteins in mammalian cells patent-application January 2010
Functional Annotation of Class I Lysyl-tRNA Synthetase Phylogeny Indicates a Limited Role for Gene Transfer journal August 2002
Adaptation of an Orthogonal Archaeal Leucyl-tRNA and Synthetase Pair for Four-base, Amber, and Opal Suppression † journal August 2003
Exploring the Limits of Codon and Anticodon Size journal February 2002
An expanded genetic code with a functional quadruplet codon journal May 2004
Four-base codons ACCA, ACCU and ACCC are recognized by frameshift suppressor sufJ journal August 1981
Properties of the lysyl-tRNA synthetase gene and product from the extreme thermophile Thermus thermophilus. journal May 1994
In Vivo Photocrosslinking with Unnatural Amino Acid Mutagenesis journal November 2002
Addition of a photocrosslinking amino acid to the genetic code of Escherichia coli journal August 2002
An Expanded Eukaryotic Genetic Code journal August 2003
Reading frame selection and transfer RNA anticodon loop stacking journal December 1987
Expansion of the genetic code: Site-directed p-fluoro-phenylalanine incorporation in Escherichia coli journal February 1998
Incorporation of non-natural amino acids into proteins journal December 2002
Incorporation of Nonnatural Amino Acids into Streptavidin through In Vitro Frame-Shift Suppression journal January 1996
Five-base codons for incorporation of nonnatural amino acids into proteins journal September 2001
Incorporation of two nonnatural amino acids into proteins through extension of the genetic code journal November 1999
Substrate recognition by class I lysyl-tRNA synthetases: A molecular basis for gene displacement journal January 1999
Complete Sequence and Gene Organization of the Genome of a Hyper-thermophilic Archaebacterium, Pyrococcus horikoshii OT3 journal January 1998
Structural basis for orthogonal tRNA specificities of tyrosyl-tRNA synthetases for genetic code expansion journal May 2003
Evolutionary relationship between Halobacterium cutirubrum and eukaryotes determined by use of aminoacyl-tRNA synthetases as phylogenetic probes journal March 1980
Expanding the genetic code: selection of efficient suppressors of four-base codons and identification of “shifty” four-base codons with a library approach in Escherichia coli journal March 2001
Generation of a Bacterium with a 21 Amino Acid Genetic Code
  • Mehl, Ryan A.; Anderson, J. Christopher; Santoro, Stephen W.
  • Journal of the American Chemical Society, Vol. 125, Issue 4, p. 935-939 https://doi.org/10.1021/ja0284153
journal January 2003
Insertions in the anticodon loop of tRNA1Gln(sufG) and tRNALys promote quadruplet decoding of CAAA journal May 2002
An efficient system for the evolution of aminoacyl-tRNA synthetase specificity journal September 2002
An archaebacteria-derived glutamyl-tRNA synthetase and tRNA pair for unnatural amino acid mutagenesis of proteins in Escherichia coli journal December 2003
Introduction of specialty functions by the position-specific incorporation of nonnatural amino acids into proteins through four-base codon/anticodon pairs journal October 2001
Functional convergence of two lysyl-tRNA synthetases with unrelated topologies journal March 2002
A New Functional Suppressor tRNA/Aminoacyl−tRNA Synthetase Pair for the in Vivo Incorporation of Unnatural Amino Acids into Proteins journal May 2000
Expanding the Genetic Code journal January 2005
Addition of the keto functional group to the genetic code of Escherichia coli journal December 2002
Actions of the anticodon arm in translation on the phenotypes of RNA mutants journal November 1986

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