Molecular genetics of myosin motors in Arabidopsis. Progress report, [July 1, 1992--February 28, 1994]
We have evidence for at least nine myosin-like genes in Arbidopsis, six of which have been cloned by a PCR-based method from genomic DNA, two have been isolated by genomic DNA cloning, and four have been identified by cDNA cloning. Most of our attention has been focused on the four myosin genes for which we have cDNA clones, and these cDNAs have now been sequenced to completion. Each of these myosins is similar in overall structure, with each containing the characteristic myosin head (motor) domain, which possesses ATP- and actin-binding motifs, a series of IQ repeats, which may be involved in calmodulin binding, a domain with a high probability of forming an alpha-helical coiled-coil secondary structure, which may allow the polypeptides to form dimers, and a variable tail domain, which may serve to define the specific cellular component that each myosin interacts with. One of these myosin genes, called MYA1, displays structural similarity to class of myosins that includes the yeast MYO2, mouse Dilute, and chicken p190 proteins, and this group of myosins is thought to play a role in intracellular trafficking of organelles. Because MYA1 is similar to this interesting class of myosins, we have chosen to conduct detailed studies of MYA1.
- Research Organization:
- Univ. of Michigan, Ann Arbor, MI (United States)
- Sponsoring Organization:
- USDOE, Washington, DC (United States)
- DOE Contract Number:
- FG02-92ER20068
- OSTI ID:
- 10159300
- Report Number(s):
- DOE/ER/20068-1; ON: DE94013697; BR: KC0600000
- Resource Relation:
- Other Information: PBD: [1994]
- Country of Publication:
- United States
- Language:
- English
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