Nucleic acids encoding antifungal polypeptides and uses thereof

Patent · Tue Nov 02 00:00:00 EDT 2010

OSTI ID:1014929

Altier, Daniel J ^[1]; Ellanskaya, I A ^[2]; Gilliam, Jacob T ^[3]; Hunter-Cevera, Jennie ^[4]; Presnail, James K ^[5]; Schepers, Eric ^[6]; Simmons, Carl R ^[7]; Torok, Tamas ^[8]; Yalpani, Nasser ^[9]

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1. Granger, IA
2. Kyiv, UA
3. Norwalk, IA
4. Elliott City, MD
5. Avondale, PA
6. Port Deposit, MD
7. Des Moines, IA
8. Richmond, CA
9. Johnston, IA

Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include an amino acid sequence, and variants and fragments thereof, for an antipathogenic polypeptide that was isolated from a fungal fermentation broth. Nucleic acid molecules that encode the antipathogenic polypeptides of the invention, and antipathogenic domains thereof, are also provided. A method for inducing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Compositions comprising an antipathogenic polypeptide or a transformed microorganism comprising a nucleic acid of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antipathogenic polypeptide of the invention are also disclosed.

• Research Organization: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC02-05CH11231
• Assignee: Pioneer Hi-Bred International, Inc. (Johnston, IA); E.I. du Pont de Nemours and Company (Wilmington, DE); The Regents of the University of California (Oakland, CA)
• Patent Number(s): 7,825,294
• Application Number: US Patent Application 12/503,952
• OSTI ID: 1014929
• Country of Publication: United States
• Language: English