Isolating human DNA repair genes using rodent-cell mutants
The DNA repair systems of rodent and human cells appear to be at least as complex genetically as those in lower eukaryotes and bacteria. The use of mutant lines of rodent cells as a means of identifying human repair genes by functional complementation offers a new approach toward studying the role of repair in mutagenesis and carcinogenesis. In each of six cases examined using hybrid cells, specific human chromosomes have been identified that correct CHO cell mutations affecting repair of damage from uv or ionizing radiations. This finding suggests that both the repair genes and proteins may be virtually interchangeable between rodent and human cells. Using cosmid vectors, human repair genes that map to chromosome 19 have cloned as functional sequences: ERCC2 and XRCC1. ERCC1 was found to have homology with the yeast excision repair gene RAD10. Transformants of repair-deficient cell lines carrying the corresponding human gene show efficient correction of repair capacity by all criteria examined. 39 refs., 1 fig., 1 tab.
- Research Organization:
- Lawrence Livermore National Lab., CA (USA); Texas Univ., Smithville (USA). Cancer Center
- DOE Contract Number:
- W-7405-ENG-48
- OSTI ID:
- 6645179
- Report Number(s):
- UCRL-96478; CONF-8703112-1; ON: DE87008735
- Resource Relation:
- Conference: Banbury Centre conference on mammalian cell mutagenesis, Cold Springs Harbor, NY, USA, 22 Mar 1987; Other Information: Portions of this document are illegible in microfiche products
- Country of Publication:
- United States
- Language:
- English
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Repair of UV damaged DNA, genes and proteins of yeast and human
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
DNA REPAIR
DNA-CLONING
GENES
GENETIC MAPPING
EXCISION REPAIR
CELL CULTURES
CHO CELLS
MAN
MUTANTS
PYRIMIDINE DIMERS
SPECIES DIVERSITY
STRAND BREAKS
ULTRAVIOLET RADIATION
ANIMAL CELLS
ANIMALS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CLONING
ELECTROMAGNETIC RADIATION
MAMMALS
MAPPING
PRIMATES
RADIATIONS
RECOVERY
REPAIR
VERTEBRATES
550200* - Biochemistry
550400 - Genetics
560200 - Thermal Effects