Repair of UV damaged DNA, genes and proteins of yeast and human
Our objectives are to determine the molecular mechanism of the incision step of excision repair of ultraviolet (UV) light damaged DNA in eukaryotic organisms, using the yeast Saccharomyces cerevisiae as a model system, as well as studying the human homologs of yeast excision repair and postreplication repair proteins. In addition to its single-stranded DNA-dependent A TPase and DNA helicase activities, we have found that RAD3 protein also possesses DNA-RNA helicase activity, and that like RAD3, the Schizosaccharomyces pombe RAD3 homolog, rhp3{sup +}, is an essential gene. We have overexpressed the human RAD3 homolog, ERCC2, in yeast to facilitate its purification. The RAD10 protein was purified to homogeneity and shown to bind DNA. ERCC3y, the yeast homolog of the human ERCC-3/XP-B gene, has been sequenced and shown to be essential for viability. The Drosophila and human homologs of RAD6, required for postreplication repair and UV induced mutagenesis, were shown to complement the rad6 {Delta} mutation of yeast. Since defective DNA repair and enhanced neoplasia characterize several human genetic diseases, and repair proteins are highly conserved between yeast and man, a thorough understanding of the molecular mechanisms of DNA repir in yeast should provide a better understanding of the causes of carcinogenesis.
- Research Organization:
- Rochester Univ., NY (USA). School of Medicine and Dentistry
- Sponsoring Organization:
- USDOE; USDOE, Washington, DC (USA)
- DOE Contract Number:
- FG02-88ER60621
- OSTI ID:
- 5763197
- Report Number(s):
- DOE/ER/60621-4; ON: DE91012170
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
EXCISION REPAIR
GENE MUTATIONS
GENE RECOMBINATION PROTEINS
FRACTIONATION
DNA SEQUENCING
ESCHERICHIA COLI
GENE OPERONS
GENE RECOMBINATION
GENES
GENETICS
NUCLEOPROTEINS
PROGRESS REPORT
SACCHAROMYCES CEREVISIAE
TRANSCRIPTION
ULTRAVIOLET RADIATION
BACTERIA
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BIOLOGY
DNA REPAIR
DOCUMENT TYPES
ELECTROMAGNETIC RADIATION
EUMYCOTA
FUNGI
MICROORGANISMS
MUTATIONS
ORGANIC COMPOUNDS
PLANTS
PROTEINS
RADIATIONS
RECOVERY
REPAIR
SACCHAROMYCES
SEPARATION PROCESSES
STRUCTURAL CHEMICAL ANALYSIS
YEASTS
550200* - Biochemistry
550400 - Genetics