Variable effects of dexamethasone on protein synthesis in clonal rat osteosarcoma cells

Journal Article · Sun May 01 00:00:00 EDT 1988 · Endocrinology; (United States)

DOI:https://doi.org/10.1210/endo-122-5-2127· OSTI ID:7154938

Hodge, B O; Kream, B E

We examined the effects of dexamethasone on protein synthesis in clonal rat osteoblastic osteosarcoma (ROS) cell lines by measuring the incorporation of (/sup 3/H)proline into collagenase-digestible and noncollagen protein in the cell layer and medium of the cultures. In ROS 17/2 and subclone C12 of ROS 17/2.8, dexamethasone decreased collagen synthesis with no change in DNA content of the cultures. In ROS 17/2.8 and its subclone G2, dexamethasone stimulated collagen and noncollagen protein synthesis, with a concomitant decrease in the DNA content of the cells. These data indicate that ROS cell lines are phenotypically heterogeneous and suggest that in normal bone there may be distinct subpopulations of osteoblasts with varying phenotypic traits with respect to the regulation of protein synthesis.

• Research Organization: Univ. of Connecticut Health Center, Farmington (USA)
• OSTI ID: 7154938
• Journal Information: Endocrinology; (United States), Vol. 122:5
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
DEXAMETHASONE
BIOLOGICAL EFFECTS
PROTEINS
BIOSYNTHESIS
CLONE CELLS
COLLAGEN
ESTRADIOL
PROGESTERONE
PROLINE
RATS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
TUMOR CELLS
ADRENAL HORMONES
AMINES
AMINO ACIDS
ANIMAL CELLS
ANIMALS
AZOLES
CARBOXYLIC ACIDS
CELL CULTURES
CORTICOSTEROIDS
ESTRANES
ESTROGENS
GLUCOCORTICOIDS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HORMONES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KETONES
LABELLED COMPOUNDS
MAMMALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PREGNANES
PYRROLES
PYRROLIDINES
RODENTS
SCLEROPROTEINS
STEROID HORMONES
STEROIDS
SYNTHESIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques