Effects of interleukin-6 on cellular function in UMR-106-01 osteoblastlike cells

Journal Article · Fri Feb 01 00:00:00 EST 1991 · Journal of Bone and Mineral Research; (USA)

DOI:https://doi.org/10.1002/jbmr.5650060206· OSTI ID:5639484

Fang, M A; Hahn, T J ^[1]

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1. Wadsworth VA Medical Center, Los Angeles, CA (USA)

High levels of interleukin-6 (IL-6) have been detected in synovial fluid from patients with inflammatory arthropathies associated with local bone resorption, suggesting a role for IL-6 as a local regulator of bone resorption and remodeling. In the present study we examined the effects of IL-6 on ({sup 3}H)thymidine (({sup 3}H)TdR) incorporation, collagen synthesis, and alkaline phosphatase activity in UMR-106-01 rat osteoblastic osteosarcoma cells. IL-6 stimulated a dose-dependent increase in ({sup 3}H)TdR incorporation that was maximal at 1000 U/ml (- 147% of basal, p less than 0.005) in osteoblastlike cells that were in a logarithmic phase of growth. The increase in ({sup 3}H)TdR incorporation was maximal between 12 and 24 h and was neutralized by pretreatment with the polyclonal rabbit antibody to IL-6. IL-6 also increased cell number and the secretion of prostaglandin E2 in UMR-106-01 cells in logarithmic growth phase. The stimulation of ({sup 3}H)TdR incorporation and release of PGE2 into the culture medium by IL-6 was inhibited by indomethacin. A 24 h exposure of the osteoblastlike cells to 1000 U/ml of IL-6 reduced ({sup 3}H)proline incorporation into collagenase-digestible (CDP) protein to 73% of control values (p less than 0.01). Noncollagen protein (NCP) synthesis was inhibited to 80% of control values (p less than 0.01) by 1000 U/ml of IL-6. The inhibitory effect was relatively greater on CDP than on NCP and consequently resulted in a decrease in the percentage of collagen synthesis. Alkaline phosphatase activity was not altered in these cells after a 24 h exposure to 1-1000 U/ml of IL-6.

• OSTI ID: 5639484
• Journal Information: Journal of Bone and Mineral Research; (USA), Vol. 6:2; ISSN 0884-0431
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ALKALINE PHOSPHATASE
ENZYME ACTIVITY
COLLAGEN
BIOSYNTHESIS
LYMPHOKINES
BIOCHEMICAL REACTION KINETICS
SKELETAL DISEASES
PATHOGENESIS
THYMIDINE
METABOLISM
BONE CELLS
PHENOTYPE
PROSTAGLANDINS
PROTEINS
RATS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
TUMOR CELLS
ANIMAL CELLS
ANIMALS
AZINES
CONNECTIVE TISSUE CELLS
DISEASES
ENZYMES
ESTERASES
GROWTH FACTORS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
HYDROLASES
ISOTOPE APPLICATIONS
KINETICS
MAMMALS
MITOGENS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOSPHATASES
PYRIMIDINES
REACTION KINETICS
RIBOSIDES
RODENTS
SCLEROPROTEINS
SOMATIC CELLS
SYNTHESIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques