Hormonal regulation of medullary bone metabolism in the laying hen

Thesis/Dissertation · Thu Jan 01 00:00:00 EST 1987

OSTI ID:6994384

Harrison, J R

A new organ culture system for the study of bone formation has been developed using medullary bone, a non-structural, metabolically active form of bone which is found in the marrow cavities of egg-laying birds. In the presence of fetal calf serum, bone explants were viable in culture by morphological criteria, and retained large numbers of osteoblasts and osteoclasts. Incorporation of /sup 3/H-proline into collagenase-digestible protein (CDP) and non-collagen protein (NCP) was determined using purified bacterial collagenase. Collagen accounted for over 10% of the total protein labeled. The calcium-regulating hormones, parathyroid hormone and 1,25-dihydroxyvitamin D3 (1,25(OH)2D3), caused a dose-dependent inhibition of /sup 3/H-proline incorporation into CDP. The effective dose range of 1,25(OH)2D3 was 0.1 nM to 100 nM, while that of PTH was 1.0 nM to 100 nM. The effect of both hormones was specific for collagen, since /sup 3/H-proline incorporation into NCP was unaffected. Hydroxyproline analysis of bone explants and culture medium revealed that both hormones decreased the total hydroxyroline content of the cultures, suggesting that the inhibition of /sup 3/H-proline incorporation into DCP is due to inhibition of collagen synthesis.

• Research Organization: Connecticut Univ., Storrs (USA)
• OSTI ID: 6994384
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
PARATHORMONE
BIOLOGICAL FUNCTIONS
PEPTIDE HYDROLASES
ENZYME ACTIVITY
PROTEINS
METABOLISM
VITAMIN D
CHICKENS
DOSE-RESPONSE RELATIONSHIPS
PROLINE
SKELETON
TRACER TECHNIQUES
TRITIUM COMPOUNDS
AMINES
AMINO ACIDS
ANIMALS
AZOLES
BIRDS
BODY
CARBOXYLIC ACIDS
ENZYMES
FOWL
FUNCTIONS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HORMONES
HYDROLASES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PEPTIDE HORMONES
PYRROLES
PYRROLIDINES
VERTEBRATES
VITAMINS
550501* - Metabolism- Tracer Techniques