Isolation of a mannose/N-acetylglucosamine receptor from rabbit lung

Journal Article · Thu May 01 00:00:00 EDT 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6992261

Lennartz, M R; Wileman, T E; Stahl, P D

The presence of a mannose receptor on alveolar macrophages was first described in 1978 and later extended to other macrophage populations. Recently the novel ligand, mannose-conjugated lactoperoxidase, was used to identify this receptor as a 175kD protein. A 175kD protein exhibiting mannose and N-acetylglucosamine (GlcNAc)-binding properties was isolated from rabbit lung membranes. Membranes were washed with high salt, mannose and EDTA to remove endogenously bound ligand and were subsequently extracted with 1% Triton-X 100. The extract was subjected to affinity chromatography on Mannose-Sepharose followed by GlcNAc-Agarose. Triton was exchanged for 1% CHAPS while the protein was bound to GlcNAc-Agarose, allowing the eluate to be concentrated without denaturation. The eluted protein bound (/sup 125/I)mannose-BSA in a mannan-inhibitable fashion. Microgram quantities of protein were isolated in this fashion. SDS-PAGE revealed a major protein band at 175kD. Amino acid analysis indicates low concentrations of methionine. Results from concanavalin A binding studies and endoglycosidase F digestion suggest that the mannose receptor is a glycoprotein containing N-linked oligosaccharides.

• Research Organization: Washington Univ. School of Medicine, St. Louis, MO
• OSTI ID: 6992261
• Report Number(s): CONF-8606151-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:6; Conference: 76. annual meeting of the Federation of American Society for Experimental Biology, Washington, DC, USA, 8 Jun 1986
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GLUCOSAMINE
RECEPTORS
BIOCHEMICAL REACTION KINETICS
PURIFICATION
CELL MEMBRANES
CHROMATOGRAPHY
CONCANAVALIN
ELECTROPHORESIS
GLUCOPROTEINS
IODINE 125
LIGANDS
LUNGS
MANNOSE
RABBITS
TRACER TECHNIQUES
AGGLUTININS
ALDEHYDES
AMINES
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BODY
CARBOHYDRATES
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
HEMAGGLUTININS
HEXOSAMINES
HEXOSES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LECTINS
MAMMALS
MEMBRANE PROTEINS
MEMBRANES
MONOSACCHARIDES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RESPIRATORY SYSTEM
SACCHARIDES
SEPARATION PROCESSES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques