Structural analysis of carbohydrate binding by the macrophage mannose receptor CD206

Feinberg, Hadar; Jégouzo, Sabine A. F.; Lasanajak, Yi; Smith, David F.; Drickamer, Kurt; Weis, William I.; Taylor, Maureen E.

doi:10.1016/j.jbc.2021.100368

Title: Structural analysis of carbohydrate binding by the macrophage mannose receptor CD206

Journal Article · Tue Feb 02 00:00:00 EST 2021 · Journal of Biological Chemistry

DOI:https://doi.org/10.1016/j.jbc.2021.100368· OSTI ID:1816764

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^[2]; Lasanajak, Yi ^[3]; Smith, David F. ^[3];

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Stanford Univ., CA (United States). School of Medicine. Depts. of Structural Biology and Molecular and Cellular Physiology
Imperial College, London (United Kingdom). Dept. of Life Sciences
Emory Univ., Atlanta, GA (United States). Emory Comprehensive Glycomics Core

The human mannose receptor expressed on macrophages and hepatic endothelial cells scavenges released lysosomal enzymes, glycopeptide fragments of collagen, and pathogenic microorganisms and thus reduces damage following tissue injury. The receptor binds mannose, fucose, or N-acetylglucosamine (GlcNAc) residues on these targets. C-type carbohydrate recognition domain 4 (CRD4) of the receptor contains the site for Ca²⁺-dependent interaction with sugars. To investigate the details of CRD4 binding, glycan array screening was used to identify oligosaccharide ligands. The strongest signals were for glycans that contain either Manα1-2Man constituents or fucosein various linkages. The mechanisms of binding to monosaccharides and oligosaccharide substructures present in many of these ligands were examined in multiple crystal structures of CRD4. Binding of mannose residues to CRD4 results primarily from interaction of the equatorial 3- and 4-OH groups with a conserved principal Ca²⁺ common to almost all sugar-binding C-type CRDs. In the Manα1-2Man complex, supplementary interactions with the reducing mannose residue explain the enhanced affinity for this disaccharide. Bound GlcNAc also interacts with the principal Ca²⁺ through equatorial 3- and 4-OH groups, whereas fucose residues can bind in several orientations, through either the 2- and 3-OH groups or the 3- and 4-OH groups. Secondary contacts with additional sugars in fucose-containing oligosaccharides, such as the Lewis-a trisaccharide, provide enhanced affinity for these glycans. These results explain many of the biologically important interactions of the mannose receptor with both mammalian glycoproteins and microbes such as yeast and suggest additional classes of ligands that have not been previously identified

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Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES); Biotechnology and Biological Sciences Research Council; National Institutes of Health (NIH)

Grant/Contract Number:: AC02-76SF00515; BB/P005659/1; P30GM133894

OSTI ID:: 1816764

Journal Information:: Journal of Biological Chemistry, Vol. 296, Issue C; ISSN 0021-9258

Publisher:: American Society for Biochemistry and Molecular BiologyCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
CD206
carbohydrate-binding protein
scavenger receptor
crystal structure
C-type lectin
glycobiology
glycoprotein

Title: Structural analysis of carbohydrate binding by the macrophage mannose receptor CD206

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References (53)

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