Increased production of cellulase of Trichoderma sp. by pH cycling and temperature profiling

Journal Article · Sat Nov 01 00:00:00 EST 1980 · Biotechnol. Bioeng.; (United States)

DOI:https://doi.org/10.1002/bit.260221104· OSTI ID:6742205

Mukhopadhyay, S N; Malik, R K

Cultivation of Trichoderma reesei QM 9414 on 3% (w/v) cellulose medium (C/N ratio equal to 8.5) produced 4.5 IU/ml cellulase in 180 hr at a cell growth of 8.0 g/liter (0.266 g cell/g cellulose). It corresponded to an average cellulase productivity 25.0 IU/liter/hr (3.5 IU/g cell/hr. In the same medium 9.5 g/liter cell mass (0.316 g cell/g cellulose), 6.2 IU/ml cellulase, and 38.75 IU/liter/hr (4.0 IU/g cell/hr) cellulase productivity could be obtained using pH cycling condition during cultivation. Cell mass, cellulase yield, and productivity were further increased to 10.0 g/liter, 7.2 IU/ml and 44.0 IU/liter/hr (4.5 IU/g cell/hr), respectively, by simultaneous pH cycling and temperature profiling strategy. Results are described.

• Research Organization: Biochemical Engineering Research Centre, Indian Institute of Technology, Delhi, Hauz Khas, New Delhi - 110029, India
• OSTI ID: 6742205
• Journal Information: Biotechnol. Bioeng.; (United States), Vol. 22:11
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
59 BASIC BIOLOGICAL SCIENCES
CELLULASE
BIOSYNTHESIS
PH VALUE
TEMPERATURE DEPENDENCE
TRICHODERMA VIRIDE
ENZYMES
FUNGI
GLYCOSYL HYDROLASES
HYDROLASES
O-GLYCOSYL HYDROLASES
PLANTS
SYNTHESIS
TRICHODERMA
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