Solid-state fermentation with Trichoderma reesei for cellulase production
Cellulase yields of 250 to 430 IU/g of cellulose were recorded in a new approach to solid-state fermentation of wheat straw with Trichoderma reesei QMY-1. This is an increase of ca. 72% compared with the yields (160 to 250 IU/g of cellulose) in liquid-state fermentation reported in the literature. High cellulase activity (16 to 17 IU/ml) per unit volume of enzyme broth and high yields of cellulases were attributed to the growth of Trichoderma reesei on a hemicellulose fraction during its first phase and then on a cellulose fraction of wheat straw during its later phase for cellulase production, as well as to the close contact of hyphae with the substrate in solid-state fermentation. The cellulase system obtained by the solid-state fermentation of wheat straw contained cellulases (17.2 IU/ml), ..beta..-glucosidase (21.2 IU/ml), and xylanases (540 IU/ml). This cellulase system was capable of hydrolyzing 78 to 90% of delignified wheat straw (10% concentration) in 96 h, without the addition of complementary enzymes, ..beta..-glucosidase, and xylanases. 29 references.
- Research Organization:
- Inst. Armand-Frappier, Laval, Quebec, Canada
- OSTI ID:
- 5480899
- Journal Information:
- Appl. Environ. Microbiol.; (United States), Vol. 49:1
- Country of Publication:
- United States
- Language:
- English
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Production of cellulase in solid-state fermentation with Trichoderma reesei MCG 80 on wheat straw
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Related Subjects
09 BIOMASS FUELS
CELLULASE
BIOSYNTHESIS
GLUCOSIDASE
STRAW
FERMENTATION
XYLANASE
CELLULOSE
CHEMICAL REACTION YIELD
ENZYMATIC HYDROLYSIS
HEMICELLULOSE
TRICHODERMA VIRIDE
BIOCONVERSION
CARBOHYDRATES
CHEMICAL REACTIONS
DECOMPOSITION
ENZYMES
FUNGI
GLYCOSYL HYDROLASES
HYDROLASES
HYDROLYSIS
LYSIS
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
PLANTS
POLYSACCHARIDES
SACCHARIDES
SOLVOLYSIS
SYNTHESIS
TRICHODERMA
YIELDS
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