Tritiated 2-deoxy-D-glucose as a probe for cell membrane permeability studies
Tritiated 2-deoxy-D-glucose was taken up and phosphorylated by cultured cells of neuronal (NIE 115), glial (138 MG), muscle (L 6) and liver (BRL 123) origin. Upon perfusion the cells slowly released 2-deoxy-D-glucose 6-phosphate. The following values for rate constants, half-lives, and activation energies for the efflux were obtained: NIE 115: 0.0048 min/sup -1/, 143 min, and 72 kJ mol/sup -1/; 138 MG: 0.0013 min/sup -1/, 547 min, and 85 kJ mol/sup -1/; L 6: 0.0022 min/sup -1/, 311 min, and 60 kJ mol/sup -1/; and BRL 123: 0.0013 min/sup -1/, 528 min and 63 kJ mol/sup -1/. When the cultures were perfused with buffer containing Triton X-100 a time- and concentration-dependent increase in the rate of efflux of 2-deoxy-D-glucose 6-phosphate was obtained. It is suggested that 2-deoxy-D-(/sup 3/H)glucose can be used as a probe in studies of general cell membrane permeability changes.
- Research Organization:
- Univ. of Stockholm, Sweden
- OSTI ID:
- 6727072
- Journal Information:
- Anal. Biochem.; (United States), Vol. 120:1
- Country of Publication:
- United States
- Language:
- English
Similar Records
Lithium prevents early cytosolic calcium increase and secondary injurious calcium overload in glycolytically inhibited endothelial cells
Temperature dependence of membrane permeability in cultured cells exposed to benzene and phenol
Related Subjects
CELL MEMBRANES
PERMEABILITY
GLUCOSE
PHOSPHORYLATION
UPTAKE
ANIMAL CELLS
BIOCHEMICAL REACTION KINETICS
CELL CULTURES
GLIOMAS
LABELLED COMPOUNDS
LIVER
MICE
MUSCLES
NERVE CELLS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ALDEHYDES
ANIMALS
BODY
CARBOHYDRATES
CELL CONSTITUENTS
CHEMICAL REACTIONS
DIGESTIVE SYSTEM
DISEASES
GLANDS
HEXOSES
ISOTOPE APPLICATIONS
KINETICS
MAMMALS
MEMBRANES
MONOSACCHARIDES
NEOPLASMS
ORGANIC COMPOUNDS
ORGANS
REACTION KINETICS
RODENTS
SACCHARIDES
SOMATIC CELLS
VERTEBRATES
550301* - Cytology- Tracer Techniques